应用高分辨率显微CT进行大鼠周围神经微血管三维可视化研究

目的 探讨采用高分辨率显微CT（Micro-CT）研究大鼠周围神经微血管三维构筑的可行性，比较两种不同的造影剂对成像效果的影响。
方法 SD大鼠40只，随机分为2组，分别灌注明胶-氧化铅和Microfil，取大鼠两侧坐骨神经行Micro-CT扫描，同时设定电压30kVP、功率40W作为
成像时的最佳能量参数。将扫描得到的断层图像转变为Dicom格式输出至个人电脑，利用Mimics 10.0软件对其进行三维重建。结果 将灌注不同
造影剂后得到的标本图片分别从大体和三维两个方面进行对照,发明明胶-氧化铅的造影效果明显优于Microfil;Micro-CT在扫描重建时呈现出低
耗时、低变化以及高清晰度的特点。结论 Microfil 在灌注微血管造影时存在者灌注压力要求高、成像质量差等缺点,而传统的明胶-氧化铅在
微血管造影上仍有其独到的优越性;Micro-CT不失为一种研究大鼠周围神经微血管三维构筑的好方法。

Three-dimensional visualization of microvessels related to the peripheral nerve by using microcomputed tomography

Objective To develop a new method for three-dimensional reconstructions of microvessels related to peripheral nerve
by using microcomputed tomography(Micro-CT), and to compare the effect of imaging between two different radiopaque materials.
Methods Forty SD rats were randomly divided into 2 groups, and then injected with lead oxide-gelatin and microfil respectively.
Sciatic nerves were scanned by Micro-CT. The best scanning parameter was set up at 30kVP and 40W. The sectional images were
transferred to Dicom format and imported to a personal computer. Three-dimensional reconstructions of microvessels were performed
by using Mimics 10.0 software. Results We compared the images of the specimens injected with different radiopaque materials,
and found that imaging effect of gelatin-lead oxide was better than microfil. Micro-CT showed that imaging of gelatin-lead oxide
was of characteristics such as low cost, low variation and high definition in scanning and reconstruction. Conclusions Microfil
has disadvantages such as strict requirements of perfusion pressure, poor imaging quality and other characteristics in
microangiography, while the traditional gelatin-lead oxide has its unique advantages in those areas. Micro-CT is a good method in
study of microvascular architecture of rat’s peripheral nerve.