皮质素对神经垂体酸性磷酸酶影响的电镜组织化学观察

本研究给大鼠肌注大剂量醋酸可的松(25 mg/d),取垂体神经部,分别用多聚甲醛-戊二醛混合固定液或粹取、分馏后的戊二醛固定,冰冻切片,硫化铅法作酸性磷酸酶的组织化学反应。Caulfied液重固定,常规电镜包埋和超薄切片,省略枸橼酸铅复杂,电镜观察。正常大白鼠垂体神经部的酸性磷酸酶(AcP)阳性反应仅见于垂体细胞和毛细血管内皮细胞,反应产物沉积于稀少的溶酶体和不发达的高尔基复合体。注射醋酸可的松后,垂体神经部的AcP阳性反应区着色增强,溶酶体增多、体大,除垂体细胞和内皮细胞外,还见AcP阳性细颗粒均匀地分布于暗垂体细胞的质突,神经分泌纤维或末梢内也可见阳性反应沉积物,随着药物用量增加,AcP阳性反应区有扩大的趋势。本文进一步讨论了皮质素对神经分泌的作用和垂体细胞在神经分泌过程中的机能意义。

ELECTRON MICROSCOPY HISTOCHEMICAL OBSERVATION OF THE EFFECT OF CORTISONE ON THE ACID PHOSPHATASEOF NEUROHYPOPHYSIS

Twenty-eight adult male rats were used for this study. The experimentar were injected intramuscular with 25 mg/d cortisone acetate and were killed in various intervals separately. The neurohypophysis were fixed in mixed solution of 4% paraformaldehyde and 2% glutaraldehyde in buffered cacodylate. Frozens ections were incubated in the medium of sodium glycero-phosphate and lead nitrate, postfixed in caulfield solution, dehydrated, embedded, ultrathin sectioned, stained With uranium acetate without lead citrate, and observed under EM, In the neurohypophysis of control animals, the active areas of acid phosphatase were few and were limited in lysosome and Golgi apparatus of pituicytes and endothelia of capillaries. After injection of cortisone, the lysosome of pituicytes were increased and the activity of acid phosphatase were strengthened. Besides pituicytes and endothelia cells, there were so many positive thin granules in the processes of dark pituicytes, some areas of positive reaction of AcP appeared in neurosecretion fibers. After increasing the drug dosage, positive reaction of AcP areas showed a tendency of expansion. In this study, we discussed the effect of cortisone on the AcP of neurohypophysis and the functional significance of pituicytes in neurosecretion procedure.