| 次声对大鼠大脑皮层神经元型NOS和诱导型NOS mRNA表达的影响 |
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| 引用本文： | 肖华胜,薛泳涛,张萍,贾克勇,程希平,黄文晋,陈镔复,陈景藻,鞠躬.次声对大鼠大脑皮层神经元型NOS和诱导型NOS mRNA表达的影响[J].第四军医大学学报,2000,21(8):954-956. |
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| 作者姓名： | 肖华胜 薛泳涛 张萍 贾克勇 程希平 黄文晋 陈镔复 陈景藻 鞠躬 |
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| 作者单位： | 1. 第四军医大学:神经科学研究所,陕西,西安,710033
2. 第四军医大学:西京医院理疗与康复科,陕西,西安,710033 |
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| 基金项目： | 总后勤部科研项目，96Z042， |
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| 摘 要： | 探讨次声作用于大鼠后，大脑皮层神经元型一氧化氮合酶（ｎＮＯＳ）和诱导型一氧化氮合酶（ｉＮＯＳ）表达的变化。方法雄性ＳＤ大鼠放入次声舱，经８Ｈｚ，１２０ｄＢ作用２ｈ，每日１次，分别于１，７和１４ｄ处死动物，取大脑皮层提取总ＲＮＡ，进行ＲＴ－ＰＣＲ，以未经次声作用的作照，检测ｎＮＯＳ和ｉＮＯＳｍＲＮＡ表达的变化。结果次声连续作用，７，１４ｄ后，ｎＮＯＳｍＲＮＡ表明显降低次声作用１ｄ后，ｉＮＯＳ开始表达
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| 关 键 词： | 次声 一氧化氮合酶 mRNA RT－PCR nNOS iNOS |
| 修稿时间： | : |
Effect of infrasound on the expression of neuron NOS and inducible NOS in hippocampus |
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| XIAO Hua-Sheng,XUE Yong-Tao,ZHANG Ping,JIA Ke-Yong,CHENG Xi-Ping,HUANG Wen-Jin,CHEN Bin-Fu,CHEN Jing-Zao,JU Gong.Effect of infrasound on the expression of neuron NOS and inducible NOS in hippocampus[J].Journal of the Fourth Military Medical University,2000,21(8):954-956. |
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| Authors: | XIAO Hua-Sheng XUE Yong-Tao ZHANG Ping JIA Ke-Yong CHENG Xi-Ping HUANG Wen-Jin CHEN Bin-Fu CHEN Jing-Zao JU Gong |
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| Abstract: | AIM To observe the effect of infrasound on the expression of neuron NOS(nNOS) and inducible NOS(iNOS) in hippocampus. METHOD Male Sprague Dawley rats were exposed to infrasound of 8 Hz~120 dB for 2 hours everyday. The rats were killed after exposure of 1 day, 7 days, 14 days respectively and the total RNA of hippocampus was isolated. RT PCR was used to study the expression of nNOS andiNOS. RESULTS the expression of nNOS of hippocampus decreased dominantly after exposure of 7 days and 14 days. But the expression of iNOS was induced after exposure of 1 days, and was still expressed after 7 days, but not expressed after 14 days. CONCLUSION The expression of nNOS of hippocampus was down regulated after exposure to infrasourd. But iNOS mRNA of hippocampus is induced by infrasound. |
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| Keywords: | infrasound nitric oxide synthesiase messenger RNA expression RT PCR |
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