| 人参总皂苷诱导K562细胞凋亡的实验研究 |
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| 引用本文： | 陈婷梅,王亚平,陈地龙,李静.人参总皂苷诱导K562细胞凋亡的实验研究[J].中草药,2003,34(3):235-237. |
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| 作者姓名： | 陈婷梅 王亚平 陈地龙 李静 |
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| 作者单位： | 1. 重庆医科大学检验系临床免疫学教研室,重庆,400016
2. 重庆医科大学组织胚胎学教研室,重庆,400016 |
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| 基金项目： | 国家自然科学基金项目 ( 396 70 880 )，重庆市中医药科学基金项目 |
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| 摘 要： | 目的：研究人参总皂苷诱导白血病细胞凋亡及其机制，为进一步开发人参总皂苷提供实验依据。方法：采用细胞体外培养，图像分析技术、流式细胞术、形态学观察与免疫细胞化学等方法，研究人参总皂苷诱导K562细胞凋亡。结果：人参总皂苷对K562细胞有增殖抑制作用，并可诱导K562细胞凋亡明显增加，同时K562细胞癌基因产物C－MYC，BCL－2表达明显降低。结论：人参总皂苷能诱导K562细胞凋亡，其机制可能与调控癌基因产物的表达有关。
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| 关 键 词： | 人参总皂苷 K562 细胞凋亡 |
| 文章编号： | 0253-2670(2003)03-0235-03 |
| 收稿时间： | 2002/10/28 0:00:00 |
| 修稿时间： | 2002年10月28 |
Experimental study on effect of apoptosis of K562 cells treated with TSPG |
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| CHEN Ting-mei,WANG Ya-ping,CHEN Di-long and LI Jing.Experimental study on effect of apoptosis of K562 cells treated with TSPG[J].Chinese Traditional and Herbal Drugs,2003,34(3):235-237. |
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| Authors: | CHEN Ting-mei WANG Ya-ping CHEN Di-long and LI Jing |
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| Institution: | CHEN Ting-mei1,WANG Ya-ping2,CHEN Di-long2,LI Jing2 |
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| Abstract: | Object To clarify the mechanism for total saponins of Panax ginseng C.A.Mey. (TSPG) inducing K562 cells to apoptosis, and to provide the theoretical basis and the experimental evidence of TSPG's clinical application. Methods By using in vitro cell culture, morphometry, flow cytometry, morphological investigation and immunocytochemistry, the effects of TSPG on apoptosis in K562 cells were studied. Results The results indicated that TSPG could inhibit the proliferation of K562 cells significantly, and induce K562 cells to apoptosis. It was also showed in the experiments that after induced by TSPG, the ratio of positive K562 cells expressing C-MYC and BCL-2 is decreased. Conclusion The mechanism for TSPG to induce K562 cells to apoptosis may be related to the expression of oncogene in K562 cells. |
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| Keywords: | total saponins of Panax ginseng C A Mey (TSPG) K562 cells apoptosis |
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