外源性IL-18基因对C6胶质瘤细胞增殖及相关基因表达的影响

目的:研究IL-18基因转染对C6胶质瘤细胞增殖活性及相关基因表达的影响. 方法:用流式细胞仪观察C6/IL-18细胞周期、增殖指数的变化;用RT-PCR,蛋白印迹、免疫细胞化学方法分析C6/IL-18细胞cyclin D1,cyclin B1,Bcl-2 mRNA及蛋白的表达. 结果:与亲代C6细胞相比,C6/IL-18细胞表现为G0/G1期细胞增多、G2/M期细胞减少,细胞增殖指数(PI)降低. C6/IL-18细胞的cyclin D1和cyclin B1,Bcl-2 mRNA及蛋白表达降低. 结论: 外源性IL-18基因可降低C6细胞的增殖活性,其机制可能与Bcl-2,cyclin B1和cyclin D1表达下调有关.

Effects of exogenous IL-18 gene on the proliferation and related gene expression in C6 glioma cells

AIM: To investigate the effects of exogenous IL-18 gene on the proliferative ability and related gene expression in transfected C6 glioma cells.METHODS: The cell cycle distributions and proliferation index of C6/IL-18 and its parental C6 cells were detected by flow cytometry.RT-PCR,immunocytochemistry and Western blot analysis were applied to test the mRNA and protein expression of cyclin D1,cyclin B1 and Bcl-2 in C6/IL18 and C6 cells respectively.RESULTS: Compared with C6 cells,C6/IL-18 cells were arrested at G0/G1 phase with fewer G2/M cells.The proliferation index of C6/IL-18 cells was lower than that of C6 cells.At both mRNA and protein levels,the Bcl-2,cyclin B1 and cyclin D1 expression in C6/IL-18 cells were decreased. CONCLUSION: Exogenous IL-18 gene could reduce the proliferative ability of C6 cells by inhibiting the progress of cell cycle and down-regulating the Bcl-2,cyclin B1 and cyclin D1 expressions.