Neonatal platelets from cord blood and peripheral blood |
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| Authors: | A.G. Sitaru S. Holzhauer C.P. Speer D. Singer A. Obergfell U. Walter |
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| Affiliation: | 1. Institute of Clinical Biochemistry and Pathobiochemistry, Central Laboratory, University of Würzberg, Würzberg, Germany;2. University Children's Hospital, University of Würzburg, Würzburg, Germany;3. University Children's Hospital, University of Würzburg, Würzburg, Germany |
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| Abstract: | The haemostatic system in neonates is different from that of adults, possibly contributing to an increased incidence of bleeding disorders, such as intracranial hemorrhage. In this study, we analyzed platelets from cord blood and peripheral blood, collected at three time points after delivery from 20 term and 37 preterm neonates as well as blood from 20 healthy adults. Platelet membrane glycoproteins (GP) were quantified and P-selectin expression and PAC-1 binding ability before and after stimulation with TRAP were analyzed by whole blood flow cytometry. We found no significant differences in neonatal platelets from cord blood and peripheral blood within the first 24?h of life. Platelets from infants less than 30 weeks of gestation expressed lower levels of GP (33271?±?9381 vs. 44085?±?17287 for GPIIIa, P?P?P?P?=?0.8) during the first 12 days of life. In conclusion, we could demonstrate that neonatal platelet reactivity increases with gestational age. |
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| Keywords: | Neonates platelets platelet reactivity |
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