Microsatellite instability in acute myelocytic leukaemia developed from A-bomb survivors |
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| Authors: | M Nakanishi K Tanaka T Takahashi T Kyo H Dohy M Fujiwara |
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| Institution: | 1. Department of Cancer Cytogenetics, Research Institute for Radiation Biology and Medicine, Hiroshima University, Kasumi l-2-3, Minami-ku, Hiroshima 734-8553, Japan.;2. Fourth Department of Internal Medicine, Hiroshima Red Cross Hospital, Hiroshima 730-0052, Japan.;3. Department of Pathology, Hiroshima Red Cross Hospital, Hiroshima 730-0052, Japan. |
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| Abstract: | Purpose : Genetic alterations, including microsatellite instability (MSI), are ultimate steps toward malignant process. To investigate MSI in A-bomb survivors, leukaemic cells were analysed from 13 acute myelocytic leukaemia patients with a history of radiation exposure and also in 12 de novo patients. Materials and methods : To assess the microsatellite changes, a fluorescent system in 10 loci (BAT40, D3S643, D5S107, IRF1, MYC, D9S171, WT1, TP53, DM, D17S855) was used. Results : MSI analysis revealed a high frequency of multiple microsatellite changes in the exposed patients (84.6%) compared with non-exposed patients (8.3%). There was a significant difference (p <0.001) between the two groups. Conclusions : These analyses clearly demonstrate that leukaemic cells from heavily exposed patients contain a number of genetic instabilities that may strongly influence the development of leukaemia among people exposed to the Hiroshima A-bomb radiation. |
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