A small fish model for quantitative analysis of radiation effects using visualized thymus responses in GFP transgenic medaka |
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| Authors: | Kouichi Maruyama Norimasa Iwanami Takako Maruyama-Hayakawa Kazutaka Doi Bing Wang |
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| Affiliation: | 1. National Institute of Radiological Sciences, National Institutes for Quantum and Radiological Science and Technology, Inage-ku, Chiba, Japan;2. maruyama.kouichi@qst.go.jp;4. National Center for Geriatrics and Gerontology, Obu, Aichi, Japan;5. Faculty of Human Sciences, Sophia University, Chiyoda, Tokyo, Japan |
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| Abstract: | AbstractPurpose: The aim of this study was to establish a new method of real-time, in vivo detection of radiation damage and recovery.Methods: The thymus was observed under fluorescent light in a green fluorescent protein transgenic medaka. After irradiation, medaka thymus images were analyzed to quantify the effects of radiation by measuring changes in thymus size. A single acute irradiation of X-rays (0–30?Gy) or heavy Fe ions (0–10?Gy) was delivered to the medaka. Images were captured 0, 1, 2, 3, 5, 7, 11, and 21 d after irradiation. Dose-response assessment was conducted to provide a direct measurement of the effects of the radiation.Conclusion: A biomonitoring system to detect the effects of radiation in real time was established. Using this system, the threshold doses for the induction of thymic atrophy by acute X-rays and Fe ions were 2–5?Gy and 0.5–1?Gy, respectively. The Relative Biological Effectiveness (RBE) of Fe-ion to X-rays was estimated to be around 3. This system may be used to evaluate the risk from concurrent exposure to hazards, such as chemicals and radiation, and for aging research. |
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| Keywords: | Thymus Oryzias latipes medaka radiation effects high linear energy transfer (LET) RBE |
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