Effects of CKMT1 on radiosensitivity of nasopharyngeal carcinoma cells |
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Authors: | Ruilong Lan Fei Huang Guangxian Zhong Ruiqing Chen Zeng Wang Junying Chen |
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Affiliation: | 1. Central Lab, First Affiliated Hospital of Fujian Medical University, Fuzhou, China;2. Fujian Key Lab of Individualized Active Immunotherapy, Fuzhou, China;3. Key Laboratory of Radiation Biology of Fujian Province Universities, Fuzhou, China;4. lanruilong123@126.com;6. Department of Orthopaedics, First Affiliated Hospital of Fujian Medical University, Fuzhou, China |
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Abstract: | AbstractPurpose: Radioresistance is an important factor for unsatisfactory prognosis in Nasopharyngeal carcinoma (NPC) patients. Ubiquitous mitochondrial creatine kinase (CKMT1) is always associated with malignancy in a variety of cancers. However, its significance in NPC progression and radiosensitivity remains unclear. The present study focused on investigating the effects of CKMT1 on NPC cell radiosensitivity.Material and methods: CKMT1 was overexpressed in NPC cell line CNE-1 or knocked out in CNE-2. Biological changes were detected after cells exposing to different doses of X-ray to determine the role of CKMT1 on NPC cell radiosensitivity.Results: CKMT1 promotes proliferation and migration in NPC cell lines CNE-1 and CNE-2. Overexpression of CKMT1 in CNE-1 cells enhanced colony formation rates, reduced G2/M phase cell cycle arrest, lowered apoptosis rate and c-PARP level, and elevated STAT3 phosphorylation level after radiation treatment. While knocking out CKMT1 using the CRISPR/Cas9 system in CNE-2 cells lowered colony formation rates, increased G2/M phase cell cycle arrest, apoptosis rates, and c-PARP levels, and decreased STAT3 phosphorylation in response to radiation treatment.Conclusions: NPC cells with higher CKMT1 exhibited lower radiosensitivity through promoting phosphorylation of STAT3. Our findings suggest that CKMT1 may be an alternative radiotherapeutic target in NPC therapy. |
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Keywords: | CKMT1 radiosensitivity nasopharyngeal carcinoma |
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