Persistence of genetic damage in mice exposed to low dose of X rays |
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Authors: | Anna Giovanetti Trivikram Deshpande Emiliano Basso |
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Affiliation: | 1. ENEA ION-IRP (Ente per le Nuove Tecnologie, l'Energia e l'Ambiente-Istituto di Radioprotezione), Research Centre Casaccia, Rome, Italygiovanetti@casaccia.enea.it;3. ENEA ION-IRP (Ente per le Nuove Tecnologie, l'Energia e l'Ambiente-Istituto di Radioprotezione), Research Centre Casaccia, Rome, Italy |
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Abstract: | Purpose:?The aim of this work was to evaluate the persistence of genetic damage in CBA/J mice treated with a single irradiation of 0.1 or 1 Gy of X rays.Materials and methods:?Peripheral blood was collected from irradiated and control mice after 30 min, 24 h, 7 days, 1, 3 and 6 months from exposure and analysed by comet assay. To investigate if the whole-body irradiation affect DNA repair, half of the sampled blood cells were in vitro-irradiated with additional 4 Gy and immediately analysed. Six months from exposure haematopoietic organs were sampled for measuring apoptotic index.Results:?In mice exposed to 1 Gy genetic damage was initially high and decreased during the experimental-time, while in the 0.1 Gy group damage, at first low, persisted and slightly increased. The 0.1 Gy-irradiated mice showed also a time-dependent increasing sensitivity to the in vitro-irradiation. Six months after whole-body irradiation, the percentage of apoptotic cells observed in haematopoietic compartments from 0.1 Gy-irradiated mice was significantly higher compared to controls and to 1 Gy mice.Conclusions:?Results demonstrated that a single exposure to low-dose might induce long-term damage. Persistence of genetic damage might have relevant implications for estimating risk for low doses. |
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Keywords: | Genomic instability ionizing radiation low dose Comet assay |
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