宫颈人乳头瘤病毒感染状况及其基因型分布

目的:探讨女性宫颈人乳头瘤病毒(HPV)感染状况及其基因亚型分布,为我国HPV感染的流行病学研究和疾病控制提供参考。方法:宫颈刷收集宫颈脱落细胞,采用DNA芯片技术同时检测18种高危型和5种低危型HPV。结果:1 132例标本中共检出HPV阳性265例(23.4%),23种HPV亚型均有检出,其中高危型HPV感染181例(15.9%),低危型33例(2.9%),多重感染81例(7.2%);高危型检出率由高到低排前6位的依次为HPV52(5.3%)、HPV16(4.5%)、HPV58(3.8%)、HPV68(1.8%)、HPV56(1.7%)、HPV18(1.6%),低危型检出率由高到低依次为HPV43(3.7%)、HPV6(1.9%)、HPV11(1.8%)、HPV42(1.1%)、HPV44(0.1%);HPV感染率以20²9岁组最高(26.2%),高危型HPV感染率以4029岁组最高(26.2%),高危型HPV感染率以40⁴9岁组最高(19.9%),但HPV感染率和高危型HPV感染率在2049岁组最高(19.9%),但HPV感染率和高危型HPV感染率在20²9岁、3029岁、30³9岁、4039岁、40⁴9岁三种人群中的差异均无统计学意义(P值均>0.05)。结论:女性宫颈HPV感染率较高,高危型HPV感染非常普遍,混合亚型感染较多见,宫颈HPV感染的优势基因型为52型,HPV基因型分布有明显的地域差异。

Infection Status and Gene Subtypes of Human Papilomavirus in Cervix

Objective：To investigate the infection and genotypes of human papilomavirus （ HPV） in cervix and provide experimental evidences for the epidemiological survey and prevention of HPV.Methods：DNA chip technology was used to detect 18 types of high risk and 5 types of low risk HPV in the cervical exfoliative cells collected by a cervix brush .Results：Among the 1 132 patients,265 cases （23.4%） were positive for the total 23 subtypes of HPV , in which 181 cases （15.9%） were high risk types,33 cases （2.9%）were low risk types and 81 cases（7.2%） infected with multiple genotypes .The most frequent types of high risk HPV were HPV 52（5.3%）, HPV16（4.5%）, HPV58（3.8%）, HPV68（1.8%）, HPV56 （1.7%） and HPV18（1.6%）. The most frequent types of low risk were HPV 43 （ 3.7%） , HPV6 （ 1.9%） , HPV11 （ 1.8%） , HPV42（1.1%） and HPV44（0.1%）.The predominant frequency of total HPV was observed in individuals between the age of 20 to 29（26.2%）, and that of high risk types did between the age of 40 to 49（19.9%）.However, the positive rate of HPV and high risk types HPV showed no sta-tistically significance among individuals on the ages of 20 -29,30 -39,40 -49 years（all P＆gt;0.05 ） .Conclusion：The high infection rate of HPV in females was observed .Infection with high risk types of HPV and multiple genotypes were common .HPV type 52 was protogene in the cer-vix.Gene subtypes of HPV was different in different regions .