| 人发角蛋白人工腱材料体外生物容性的实验研究 |
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| 引用本文： | 陆声,杨海伟,李其训,林月秋,徐永清.人发角蛋白人工腱材料体外生物容性的实验研究[J].中国医师杂志,2005,7(6):759-761. |
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| 作者姓名： | 陆声 杨海伟 李其训 林月秋 徐永清 |
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| 作者单位： | 1. 成都军区昆明总医院骨科,云南,昆明,650032
2. 成都军区联勤部卫生部 |
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| 摘 要： | 目的研究人发角蛋白人工腱材料(HHKAT)的体外生物相容性。方法在体外培养的人胚腱细胞(HETC)中加入不同浓度的HHKAT浸泡液(100％、50％、10％)，培养1、3、5、7d，通过倒置显微镜，HETCI型胶原的表达，HHKAT与HEIC的复合生长情况及超微结构的观察等方法，了解HHKAT对HETC的生长及超微结构的影响。结果不同浓度HHKAT浸泡液对HEIC的形态、Ⅰ型胶原的表达及超微结构无抑制作用，并能与HHKAT粘附生长。结论HHKAT具有良好的生物相容性。
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| 关 键 词： | 人工腱材料 人发角蛋白 实验研究 HHKAT 生物相容性 超微结构 不同浓度 人胚腱细胞 倒置显微镜 体外培养 I型胶原 抑制作用 Ⅰ型胶原 浸泡液 生长 表达及 |
| 修稿时间： | 2004年11月15 |
An Experimental Study on Biocompatibility of Human Hair Keratin Artificial Tendon Material |
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| LU Sheng,LI Qi-xun,LIN Yue-qiu,et al..An Experimental Study on Biocompatibility of Human Hair Keratin Artificial Tendon Material[J].Journal of Chinese Physician,2005,7(6):759-761. |
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| Authors: | LU Sheng LI Qi-xun LIN Yue-qiu |
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| Institution: | LU Sheng,LI Qi-xun,LIN Yue-qiu,et al. Department of Orthopaedics,Kunming General Hospital,Chengdu Military Region,PLA,Kuming 650032,China |
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| Abstract: | Objective To study the biocompatibility of human hair keratin artificial tendon(HHKAT) material in vitro. Methods Human embryonic tendon cell (HETC) was incubated with various concentrations (100%,50%,10%) of extracted liquid of HHKAT material for 1,3,5 and 7 days, respectively. Collagen I expression in HETC was detected by immunocytochemistry. The effects of HHKAT material on the proliferation and ultrastructure of HETC were observed. Results Various concentrations of extracted liquid of HHKAT had no effect on the morphologic, collagen I expression and ultrastructure of HETC. HETC could adhere, grow and proliferate on the surface of HHKAT material. Conclusion HHKAT is a medical biomaterial with good biocompatibility in vitro. |
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| Keywords: | Biocompatibility Artificial tendon Tissue engineering |
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