神经生长因子对小鼠颈上神经节和大鼠腓神经损伤后修复的影响

目的 应用形态学方法观察研究神经生长因子（NGF）对长春新碱（VCR）损伤的小鼠颈上神经节和钳夹损伤的大鼠腓神经修复再生作用。方法 颈上神经节：出生2d的昆明种小鼠随机分为实验组、对照组和空白对照组。实验组皮下注射VCR（0.2mmol/L～10ul/g体重）和NGF（2、5、10ug/g体重），对照组仅注射VCR，每天1次，连续4d。光镜下测量颈上神经节横径并且观察神经节细胞形态变化。周围神经：将钳夹损伤腓神经的SD大鼠随机分为实验组和对照组，并从损伤后第1天在近损伤处分别肌注NGF（2、4、8ug/kg体重）和生理盐水，每天1次，连续12d。取腓神经和趾长伸肌，光镜观察，并计数损伤处远端和近端神经纤维数量。结果 VCR可损伤颈上神经节，神经节横径缩小，节细胞凋亡解体；NGF则可改善VCR的损害作用，神经节横径增大61%～95%，节细胞数明显增多59%～70%，细胞凋亡现象显著减轻，改善程度与NGF剂量相关。NGF对排神经再生和趾长伸肌形态变化也有明显改善作用，尤以大剂量NGF的作用更显著。结论 NGF对长春新碱损伤的小鼠颈上神经节和经钳夹损伤的大鼠腓神经有明显的促修复作用。

Study on the RegenerationEffects of NGF on Destructive Superior Cervical Ganglia of Newborn Mice and Peroneal Nerveof Adult Rats

Purpose The regeneration effects of nerve growth factor(NGF)on superior cervical ganglia innewborn mice destroyed by vincristine(VCR)and peroneal nerve of adult rats destroyed by grip were studiedby morphological methods. Methods Superior cervical ganglia. The Qunming newborn mice at 2 dayswere divided into 3 groups: experiment, control and blank. The experiment animals were injected with VCR,10 μl/g of body weight at a concentration of 0.02 mmol/L. Simultaneously, the NGF was injected 2,5,10μg/g of body weight, respectively. But the control animals were only injected with VCR at the same dose.The blank control animals weren' t treated anything. All of these chemicals were injected once a day for 4days. 24h after the last injection, the superior cervical ganglia were dissected out and analyzed their size andmorphology. Peripheral nerve. The peroneal nerve of SD adult rats were destroyed by grip, and divided into2 groups: experiment and control. The experiment rats were injected with NGF 2,4 and 8 μg/kg of bodyweight respectively, near the gripped nerve,once a day for 12 days after 24 h of the injury. 24 h after the lastinjection, the perone al nerve and extensor longus digitorum were dissected out and analyzed their morphologyand counted the number of nerve fiber at proximal and distal injury. Results VCR injection in newbommice produced severe atrophy of superior cervical ganglia. And the neuronal cells apoptosed and decomposed.Simultaneous injections of NGF prevented the noxious effects of VCR, and resulted in an increase intransverse diameter from 61 to 95 percent and the total number of neuronal cells from 59 to 70 percent. Thisimproved degree was related to the dose of NGF. Furthermore, NGF obviously improved the structure of peroneal nerve and extensor longus digitorum. And this effect was the best in the high dosage. ConclusionsNerve growth factor has an obvious regeneration effects in superior cervical ganglia of newborn mice destroyed by VCR and peroneal nerve of rat destroyed by grip.