β射线诱导成纤维细胞凋亡与防治瘢痕增生的关系

目的 探讨β射线防治瘢痕增生的作用机理。方法 用ＭＴＴ法分析β射线对增生性瘢痕成纤维细胞生长的影响。用电镜，ＤＮＡ凝胶电冰和流式细胞仪观察细胞形态和ＤＮＡ的变化。结果 β射线明显抑制增生性瘢痕成纤维细胞的生长；１０Ｇｙβ射线诱导出典型的细胞凋亡特征，如凋亡小体，ＤＮＡ梯状条带，特征性亚Ｇ１峰等，２０Ｇｙβ射线导致细胞坏死。结论 β射线诱导增生性瘢痕成纤维细胞凋亡是放射防治瘢痕增生的重要机理之一，β

The role of beta-ray induced fibroblasts apoptosis on the inhibition of hypertrophic scar]

OBJECTIVE: To investigate the role of beta-ray induced apoptosis of the fibroblasts on the inhibition of hypertrophic scar. METHODS: A series of patients with hypertrophic scar were grouply treated with a certain dose beta-ray. A liopic sample was taken in each patients. Its was then examined with MTT[3-(4,5-dimethylthiazol.-2-yl)-2,5-dipheny1 terazolium-bromide] technique for deciding the inhibition rate of the fibroblasts, with electron microscope technique, DNA agarose gel electrophoresis and flow cytometry for evaluating the morphological and biochemical characteristics of the fibroblast. RESULTS: The beta-ray radiation resulted in significant inhibition of the fibroblast. The features of fibroblast apoptosis(e.g.: apoptotic bodies, DNA ladders band and hypodiploid DNA peak) could be seen in the group with lower does (10 Gy). The features of fibroblast necrosis could also be found in the group with higher does(20 Gy). CONCLUSIONS: The beta-ray radiation could have the effect for inhibiting the proliferation of scar-derived fibroblast, but with dose dependence. It could be used for the treatment and(or) the prevention of hypertrophic scar.