虫草多糖预先给药对大鼠内毒素性急性肺损伤的影响 |
| |
引用本文: | 陈洁,徐旋里,钟泰迪,王平. 虫草多糖预先给药对大鼠内毒素性急性肺损伤的影响[J]. 中华麻醉学杂志, 2004, 29(1): 565-567. DOI: 10.3760/cma.j.issn.0254-1416.2009.06.023 |
| |
作者姓名: | 陈洁 徐旋里 钟泰迪 王平 |
| |
作者单位: | 浙江大学邵逸夫临床医学研究所,浙江大学医学院附属邵逸夫医院麻醉科,310016;浙江大学附属第一医院呼吸内科,310016; |
| |
摘 要: | Objective To evaluate the effects of Cordyceps polysaccharide (CP) pretreatment on endotoxin-induced acute lung injury (ALI) in rats.Methods Forty adult male SD rats were randomly divided into 5 groups ( n = 8 each) : CP1-3 group, ALI group and control group (group C). Group CP1-3 received CP 1, 2 and 3 g/kg by intragastric gavage every 12 h for 6 times respectively, and then received LPS 5 mg/kg iv through the femoral vein 2 h after the last administration. Croup ALI received normal saline 1 ml/100 g instead of CP, and the rest methods were the same as those described in group CP. Group C received normal saline 1 ml/100 g instead of CP and LPS, and the rest methods were the same as those described in group CP. The animals were killed 6 h after injection of LPS. The lungs were removed for determination of W/D lung weight ratio, pulmonary permeability index (PPI), WBC and PMN counts in broncho-alveolar lavage fluid (BALF) , and levels of TNF-α in lung tissues and BALF, and microscopic examination.Results Compared with group C, W/D lung weight ratio, PPI, WBC and PMN counts in BALF, lung pathological scores and levels of TNF-α in lung tissues and BALF were significantly increased in group ALI and CP1-3 ( P < 0.05 or 0.01). No significant differences were found in the indices mentioned above between group CP1 and group ALI ( P > 0.05) . The indices mentioned above were significantly lower in group CP2,3 than in group ALI ( P < 0.05) . PMN counts in BALF, lung pathological scores and levels of TNF-α in lung tissues and BALF were significantly lower in group CP3 than in group CP2 ( P < 0.05) . Conclusion Cordyceps polysaccharide pretreatment can attenuate LPS-induced ALI in a dose-dependent manner through inhibition of inflammatory response in rats.
|
关 键 词: | 虫草素 多糖类 呼吸窘迫综合征,成人 内毒素类 |
Effect of Cordyceps polysaccharide pretreatment on endotoxin-induced acute lung injury in rats |
| |
Abstract: | |
| |
Keywords: | CORDYCEPINPolysaccharidesRespiratory distress syndrome adultEndotoxins |
|
|