食管鳞癌组织血管生成拟态和HIF-1α表达及其生物学意义的探讨

目的:探讨血管生成拟态(VM)和低氧诱导因子-1α(HIF-1α)在食管鳞癌组织中的表达及意义.方法:收集临床病理资料完整的食管鳞癌组织170例,进行PAS及CD34双重染色,观察食管鳞癌中是否存在VM;免疫组化SP法检测HIF-1α蛋白的表达,并计数微血管密度(MVD).结果:在食管鳞癌组织中VM和HIF-1α表达阳性率分别为12.4％(21/170)、64.1％(109/170),显著高于正常食管黏膜组织,P＜0.01.VM在低分化食管鳞癌组织中(25.9％)高于高-中分化组(9.8％),与TNM分期、淋巴结转移和MVD有关,P＜0.05.HIF-1α的表达也与TNM分期、淋巴结转移及MVD有关,P＜0.05；VM与HIF-1α蛋白表达相关,r=0.206,P＜0.05.结论:在食管鳞癌组织中存在VM,HIF-1α高表达可能促进VM.VM与HIF-1α高表达可能是食管鳞癌浸润、转移重要生物学标志.VM与HIF-1α联合检测对食管鳞癌的进展及预后判断有重要意义.

Vasculogenic mimicry and HIF-1alpha expression in esophageal squamous cell carcinoma and their significance

OBJECTIVE: To explore the espression of vasculogenic mimicry(VM) and hypoxia-inducible-factor-1α(HIF-1α) in esophageal squamous cell carcinoma(ESCC) tissues and their significance.METHODS:Totally 170 ESCC cases with complete clinical pathology data were collected.The presence of VM was observed by immunohistochemical and histochemicaldouble staining of CD34 and PAS,and by SP immunohistochemical detection of HIF-1α protein expression,and counted MVD.RESULTS:In 170 cases of ESCC,21(12.4%) exhibited evidence of VM and 109(64.1%) were positive for HIF-1α,which were significantly higher than that of normal esophageal tissue(P<0.05).VM in lowly differentiated esophageal squamous cell carcinoma(25.9%) was higher than that in moderately to well differentiated(9.8%) and it was significantly related to TNM stage,lymph-node metastasis and MVD(P<0.05).The expression of HIF-1α protein was also related to the TNM stage,lymph node metastasis and MVD(P<0.05).Vasculogenic mimicry was related to expression of HIF-1α(r=0.206,P<0.05).CONCLUSIONS:VM exists in ESCC.Overexpression of HIF-1α may induce the formation of VM channels.VM and over-expression of HIF-1α may be important biologicalmarkers for invasion and metastasis of ESCC.The combined detection of VM and HIF-1α has an important role in predicting the progression and prognosis of ESCC.