siRNA靶向沉默hyrdC基因对胃癌细胞SGC-7901生长作用的研究

目的:观察RNA干扰靶向沉默hyrdC基因对胃癌SGC-7901细胞生长的影响.方法:将已成功构建的携带hyrdC基因以及沉默hyrdC基因的重组腺病毒Ad-hyrdC、Ad-shyrdC分别转染胃癌SGC-7901细胞,台盼蓝细胞计数和MTT法观察各组胃癌细胞生长情况,将胃癌细胞接种裸鼠皮下构建裸鼠荷瘤模型观察各组胃癌瘤体的生长情况.结果:Ad-shyrdC组细胞生长明显慢于Ad-hyrdC、Ad-null和正常对照组,差异有统计学意义,P=0.02；Ad-shyrdC组细胞的MTT值明显低于Ad-hyrdC、Ad-null和正常对照组,P=0.03；Ad-shyrdC组胃癌瘤体生长率较Ad-hyrdC、Ad-null组以及正常对照组明显减慢,P=0.02.结论:hyrdC基因具有促进胃癌细胞生长的功能,而沉默hyrdC基因可抑制胃癌细胞的生长,hyrdC基因有望成为胃癌基因治疗领域的新靶点.

Inhibitory effect of siRNA targeted silencing hyrdC gene on growth of gastric cancer cell lines SGC-7901

OBJECTIVE:To observe the effect on growth of gastric cancer cell lines SGC-7901 induced by RNAi targeted silencing hyrdC gene.METHODS:The constructed recombinant adenovirus Ad-hyrdC mediated hyrdC gene and Ad-shyrdC knocked hyrdC gene were transfected to gastric cancer SGC-7901 cell line,the cell growth was investigated by trypan blue cell count and MTT assay in vitro,nude mice model was construted by inoculating gastric cancer cell subcutaneously into nude mice in vivo,and the tumor growth of each group was investigated.RESULTS: The cell growth of Ad-shyrdC group were significantly slower as compared to Ad-shyrdC,Ad-null and control group(P=0.02);The MTT value of Ad-shyrdC group were significantly lower as compared to Ad-hyrdC,Ad-null and control group(P=0.03);The tumor growth rate of Ad-shyrdC group was significantly lower as compared to Ad-hyrdC,Ad-null and control group(P=0.02).CONCLUSION: HyrdC gene have function of promoting the gastric cancer cell growth,the knock-out of hyrdC gene can inhibit the growth of gastric cancer cell,which can become a new promising target for gastric cancer gene therapy.