益气活血法对脑缺血再灌注大鼠MAP-2及NOGO-A蛋白表达的影响

目的：探讨益气活血法对缺血再灌注大鼠的神经保护作用。方法：动物随机分为假手术组、模型组、益气组、活血组、益气活血组,以线栓法阻塞大鼠左侧大脑中动脉复制局灶性脑缺血再灌注模型,缺血2 h再灌注3、7、14 d,用免疫组织化学染色SABC法分别检测MAP-2及NOGO-A蛋白的表达。结果：脑缺血再灌注损伤后,模型组MAP-2表达下降,与假手术相比有显著性差异（P〈0.05）,而益气法、活血法和益气活血法三组治疗组均能显著促进MAP-2的表达（P〈0.01）。脑缺血再灌注损伤7 d时,模型组NOGO-A表达与假手术相比有显著性差异（P〈0.05）。而益气法、活血法和益气活血法三组治疗组NOGO-A的表达与模型组无显著性差异（P〉0.05）。结论：益气活血法能显著促进MAP-2表达,有利于脑缺血后突触重塑和神经细胞轴突的再生,促进脑卒中后神经功能恢复。

Effects of Method of Supplementing Qi and Activating Blood Therapy on Expressions of MAP-2 and NOGO-A Proteins in Rats after Cerebral Ischmia/Reperfusion Injury

Objective ： To investigate the neuroprotective effect on ischemia reperfusion rats by the method of supplementing qi and activating blood therapy. Methods ： The rats were divided into sham- operated group, model group, Yiqi group, Huoxue group, and Yiqihuoxue group randomly. MCAO model rats were made . At 3 d, 7 d and 14 d of reperfusion after 2 h, MCAO, MAP-2 and NOGO-A protein expressions were detected with immunohistochemieal SABC method respectively. Results ： After cerebral ischemia reperfusi0n dnjury, MAP=2 expression was decreased significantly in model group compared with that in sham-operated group （P〈0.05）, while three treatment groups all can significantly improve MAP-2 expression （ P〈0.01 ）. NOGO-A expression started to increase significantly in model group compared with that in sham-operated group at days （P〈0.05）, but the expression had no significant difference between three treatment groups and model group at 7 days （ P〉0.05 ）. Conclusion ： The method of supplementing qi and activating blood therapy can significantly improve MAP-2 expression and be helpful for nerve regeneration after cerebral ischemia injury.