N,O-羧甲基壳聚糖与磷酸三钙复合体的理化性能研究

目的：研究N，O-羧甲基壳聚糖（N，O-carboxymethyl chitosan，N，O-CMCS）／磷酸三钙（tficalcium phosphate，TCP）以不同质量比例复合制作骨支架材料的理化性能。方法：N，O-CMCS与TCP分别以质量比2：1、1：1和1：2混合，以同体积的水将材料调匀．制成复合体，应用红外光谱仪测试复合材料的特征谱带，扫描电镜观察复合材料的微观特征，万能材料实验机测试材料复合后15min和12h时的抗压强度和弹性模量。结果：3种复合材料中，N，O-CMCS的-COO-和-NH2的特征峰渐向低波数方向明显位移，-COO和-NH2与TCP的Ca^2＋发生了络合反应。N,O-CMCS／TCP呈三维结晶多孔结构，N，O-CMCS含量增多．复合材料孔隙增加，抗压强度变大：而TCP含量增多，其孔隙减少，弹性模量变大，刚性加强。结论：N，O-CMCS具有络合钙粒子的特性，与TCP复合后形成的复合材料具有良好的可塑性、三维多孔结构和力学性能，有望成为一种有应用前景的骨修复材料。

The characterization of scaffold made of N,O-carboxymethyl chitosan and tricalcium phosphate

PURPOSE: To study the mechanical and chemical characterization of scaffold made of N,O-carboxymethyl chitosan (N,O-CMCS) and tricalcium phosphate(TCP) with various weight ratios. METHODS: Three composites of N,O-CMCS/TCP with weight ratios of 2:1,1:1 and 1:2 were mixed using the same volume of distilled water. The characteristic bands, morphologic structure, compressive strength and Young modulus of the composites were analyzed by infrared spectroscopy (IR),scanning electron microscopy(SEM) and universal testing machine respectively. RESULTS: The shift of IR characteristic bands of -COO(-)and -NH(2) to lower wave number was observed in three composites gradually, which resulted from the complexation of -COO(-) and -NH(2) with calcium ions. The pores of the three-dimensional porous structure and compressive strength of the composite increased when there were more N,O-CMCS in the composite. When there were more TCP in the composite, its porosity became less and Young modulus increased, so that the composite demonstrated more rigidity. CONCLUSIONS: N,O-CMCS had the capability of complexing with calcium ions. The scaffold made of N,O-CMCS and TCP had the properties of three-dimensional porous structure, flexible remolding and mechanical strength, so it will be a promising biomaterial for bone substitute.