| 表皮生长因子增强大肠癌细胞Caco-2对5-FU的敏感性 |
| |
| 引用本文： | 叶霖,王国斌,陶凯雄. 表皮生长因子增强大肠癌细胞Caco-2对5-FU的敏感性[J]. 华中科技大学学报(医学版), 2010, 39(1). DOI: 10.3870/j.issn.1672-0741.2010.01.030 |
| |
| 作者姓名： | 叶霖 王国斌 陶凯雄 |
| |
| 作者单位： | 华中科技大学同济医学院附属协和医院腹腔镜外科,武汉,430022 |
| |
| 基金项目： | 湖北省卫生厅科研基金资助项目 |
| |
| 摘 要： | 目的 通过观察表皮生长因子(EGF)与5-氟尿嘧啶(5-FU)联合使用对大肠癌细胞Caco-2的作用效果,进一步研究EGF潜在的抗肿瘤特性.方法 通过MTT比色法检测EGF、5-FU及其联合使用对肿瘤细胞生长的影响,同时Western blot检测EGF对肿瘤增殖细胞核抗原(PCNA)表达的影响,以及流式细胞周期检测分析其对细胞周期变化的影响. 结果 EGF能够诱导肿瘤细胞增殖,随着其浓度的升高,G0期细胞明显减少,PCNA表达明显增加,100 ng/mL和1 000 ng/mL组与对照组比较差异具有统计学意义(均P＜0.05),并且联合治疗组对肿瘤细胞的抑制率明显高于单独使用5-FU组(P＜0.05).结论 EGF与5-FU联合使用增加了化疗敏感性,可能与其诱导静止期细胞增殖有关.
|
| 关 键 词： | 大肠癌 化疗敏感性 细胞增殖 |
Enhancement of Drug Sensitivity to 5-FU in Human Colorectal Cancer Cell Caco-2 by EGF Stimulation |
| |
| Ye Lin,Wang Guobin,Tao Kaixiong. Enhancement of Drug Sensitivity to 5-FU in Human Colorectal Cancer Cell Caco-2 by EGF Stimulation[J]. Journal of Huazhong University of Science and Technology(Health Sciences), 2010, 39(1). DOI: 10.3870/j.issn.1672-0741.2010.01.030 |
| |
| Authors: | Ye Lin Wang Guobin Tao Kaixiong |
| |
| Affiliation: | Ye Lin,Wang Guobin,Tao Kaixiong Department of Laproscopic Surgery,Union Hospital,Tongji Medical College,Huazhong University of Science , Technology,Wuhan 430022,China |
| |
| Abstract: | Objective By observing the effectiveness of epidermal growth factor(EGF)combined with 5-flauouracil(5-FU)in the treatment of human colorectal cancer,to explore the anti-tumor effects of EGF.Methods MTT assay was used to evaluate the effects of EGF and 5-FU alone or combined use on the growth of tumor cells.The effects of EGF on PCNA expression were detected by Western blot,and flow cytometry was done to measure the changes in cell cycle.Results EGF could induce proliferation of tumor cells.With the increase... |
| |
| Keywords: | colorectal cancer sensitivity to chemotherapy cell proliferation |
| 本文献已被 CNKI 万方数据 等数据库收录！ |
|
