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| 红车轴草提取物中异黄酮定量方法的研究 | |
| 引用本文： | 边清泉,杨振萍,马美仲,李松. 红车轴草提取物中异黄酮定量方法的研究[J]. 中国中药杂志, 2003, 28(11): 1044-1047 |
| 作者姓名： | 边清泉 杨振萍 马美仲 李松 |
| 作者单位： | 1. 绵阳师范学院,四川,绵阳,621000 2. 西南科技大学,四川,绵阳,621000 3. 四川大学,四川,成都,610000 |
| 基金项目： | 四川教育厅重点自然科学研究项目 ( 2 0 0 2A 1 1 8) |
| 摘 要： | 目的 :对红车轴草提取物中异黄酮含量测定方法进行研究。方法 :反向高效液相色谱法进行含量测定 ,C18反相柱 ,MeOH CH3CN 0.1%H3PO4 为流动相 ,检测波长 260nm。结果 :红车轴草提取物水解后的 4种苷元组分获良好分离 ,进样量 0.024～0.336μg与峰面积均成线性关系 ,异黄酮总含量测定结果重现性RSD =1.1%。结论 :为红车轴草提取物及制品提供一种简便、可靠、有效的质控方法。 |
| 关 键 词： | 红车轴草 高效液相色谱法 异黄酮 含量测定 |
| 文章编号： | 1001-5302(2003)11-1044-04 |
| 收稿时间： | 2003-02-20 |
| 修稿时间： | 2003-02-20 |
Study of the Methods of Determining the Isoflavones in Herbs of Trifolium pratense Extractive |
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| BIAN Qing-quan ;YANG Zhen-ping ;MA Mei-zhong ;LI Song. Study of the Methods of Determining the Isoflavones in Herbs of Trifolium pratense Extractive[J]. China Journal of Chinese Materia Medica, 2003, 28(11): 1044-1047 | |
| Authors: | BIAN Qing-quan YANG Zhen-ping MA Mei-zhong LI Song |
| Affiliation: | Mianyang Teacher's College, Mianyang 621000, Sichuan, China. Biam-55@sohu.com |
| Abstract: | OBJECTIVE: To study the methods of determining the amount of Isoflavones in red clover extractive. METHOD: RP-HPLC is employed to determine the Isoflavones, with a C18 RP Column, a moble phase of MeOH-CH3CN-0.1%H3PO4 and a detection wavelength of 260 nm. RESULT: After being water-solved, the four groups of flavoes elements satisfactorily separated, the amount of feeding in a range of 0.024-0.336 microg which has a good linear relationship with the peaks, and the total isoflavones determining results repetition, RSD=10.1%. CONCLUSION: A simple, reliable and effective quality-control method is given. |
| Keywords: | Trifolium pratense HPLC isoflavones contents determination |
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