| 人肺腺癌肿瘤相关巨噬细胞的活化表型鉴定 |
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| 引用本文： | 章必成,赵勇,王俊,郭燕,饶智国,高建飞.人肺腺癌肿瘤相关巨噬细胞的活化表型鉴定[J].武汉大学学报(医学版),2009,30(5). |
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| 作者姓名： | 章必成 赵勇 王俊 郭燕 饶智国 高建飞 |
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| 作者单位： | 1. 广州军区武汉总医院肿瘤科,湖北,武汉,430070
2. 济南军区总医院肿瘤科,山东,济南,250031 |
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| 基金项目： | 湖北省卫生厅科研项目(编号:JX3B37) |
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| 摘 要： | 目的:鉴定人肺腺癌肿瘤相关巨噬细胞(TAMs)的活化表型。方法:分别以CD68/巨噬细胞甘露糖受体(MMR)或CD68/诱导型一氧化氮合酶(i NOS)双阳性作为巨噬细胞发生替代性活化或经典活化的标记;收集40例人肺腺癌新鲜组织,双标免疫荧光染色和激光共聚焦显微镜观察TAMs表达CD68和MMR(或i NOS)。以IL-4处理人巨噬细胞,RT-PCR和Western-blot分别检测其表达MMR和i NOS的情况。结果:40例人肺腺癌全部同时表达CD68和MMR,其中CD68/MMR双阳性细胞占CD68阳性细胞的79.7%;仅有3例同时表达CD68和i NOS,CD68/i NOS双阳性细胞占CD68阳性细胞的12.4%。IL-4处理后,人巨噬细胞显著表达MMR而不表达i NOS。结论:人肺腺癌TAMs可能呈现出替代性活化的表型特征。
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| 关 键 词： | 肿瘤相关巨噬细胞 活化表型 肺腺癌 |
Activated Phenotypes Identification of Tumor-Associated Macrophages in Human Lung Adenocarcinoma |
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| ZHANG Bicheng,ZHAO Yong,WANG Jun,GUO Yan,RAO Zhiguo,GAO Jianfei.Activated Phenotypes Identification of Tumor-Associated Macrophages in Human Lung Adenocarcinoma[J].Medical Journal of Wuhan University,2009,30(5). |
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| Authors: | ZHANG Bicheng ZHAO Yong WANG Jun GUO Yan RAO Zhiguo GAO Jianfei |
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| Institution: | ZHANG Bicheng1,ZHAO Yong1,WANG Jun2,GUO Yan2,RAO Zhiguo1,GAO Jianfei11Dept.of Oncology,Wuhan General Hospital,Guangzhou Comm,,People's Liberation Army,Wuhan 430070,China2 Dept.of Oncology,General Hospital of Jinan Comm,,Jinan 250031,China |
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| Abstract: | Objective: To identify the activated phenotypes of tumor-associated macrophages(TAMs) in human lung adenocarcinoma.Methods: Co-expressions of CD68/macrophage mannose receptor(MMR) and CD68/inducible nitric oxide synthase(iNOS) were used as the markers of alternatively activated macrophages(aaMphi) and classically activated macrophages(caMphi),respectively.The activated phenotypes of TAMs were detected by double-labelled immunofluorescence staining and observed by laser confocal microscopy in 40 cases of hum... |
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| Keywords: | Tumor-Associated Macrophages Activated Phenotype Lung Adenocarcinoma |
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