外周血Tg mRNA的检测及诊断甲状腺癌的临床意义

目的：探讨检测外周血中甲状腺球蛋白mRNA对甲状腺癌的诊断价值。方法：应用逆转录聚合酶链反应（RT－PCR）技术，对55例患者（39例甲状腺癌，7例甲状腺良性肿瘤，9例非甲状腺肿瘤）进行循环血中甲状腺球蛋白mRNA检测，39例甲状腺癌中34例乳头状癌，2例滤泡癌，3例髓样癌。结果：39例中12例发现Tg mRNA（31％），其中11例为乳头状癌，1例滤泡癌，7例甲状良性肿瘤和9例非甲状腺肿瘤未发现Tg mRNA 。40％颈淋巴结转移和100％远处转移的病例Tg mRNA阳性，而无转移的病例无论原发肿瘤切除与否Tg mRNA均阴性。结论：外周血中Tg mRNA可作为甲状腺癌的肿瘤标志物，特别对颈部或远处转移具有一定的临床诊断价值。

The Significance of Expression of Tumor Marker in PeripheraL BLood of Patients with Thyroid Carcinoma

Objective:To study the diagnostic value of circulating thyroglobulin(Tg)mes-senger ribonucleic acid(mRNA)in peripheral blood in patients with thyroid carcinoma.Meth-ods:The expression of Tg mRNA was determined in peripheral blood of55patients including thyroid cancer39(35papillary,2follicular and3medullary),benign thyroid disease7and non-thyroid tumor patients9using RT-PCR assay.Results:Of 39thyroid cancer patients,Tg mR-NA was positive in12cases(31%)including papillary carcinoma 11,follicular1.Tg mRNA was positive in40%of patients with cervical metastases and100%with distant metastases.Tg mRNA was not detected in any patients without metastasis whether the tumor of thyroid was re-sected or not.Conclusion:We have demonstrated that Tg mRNA can be detected in peripheral blood of thyroid carcinoma patients especially in patients with cervical or distant metastases.