宫内急性缺血及再灌注对胎鼠肾SOD和MDA的影响

目的利用大鼠宫内急性缺血及再灌注模型检测胎龄21d鼠肾损伤后超氧化物歧化酶(SOD)、丙二醛(MDA)的变化,以探讨宫内缺血/再灌注时肾损伤的发病机制。方法选取孕21dWistar大鼠,腹腔麻醉后,暴露双角子宫及供应子宫和卵巢的动静脉血管,钳夹其双角子宫一侧动静脉血管,钳夹时间分别为10、30min,达要求时间后取下动脉夹行再灌注,再灌注时间分别为0.5、2、6、24h,对侧未钳夹宫角内的胎鼠为对照组,每个时间点留取7份标本。分别检测各个时间点胎鼠肾SOD、MDA的含量。结果①SOD活性:缺血10min时SOD活性开始降低(P>0.05),缺血30min时SOD活性下降明显(P<0.05),随再灌注时间的延长,SOD活性逐渐降低,于再灌注6h时达最低(P<0.01),而后SOD活性开始升高,再灌注24h时虽未恢复正常,但与假手术组无差异(P>0.05);缺血10min组与缺血30min组相比,后组明显低于前组,尤为再灌注0.5h和再灌注6h(P<0.01);②MDA含量:缺血10minMDA含量即开始升高(P>0.05),缺血30min时MDA含量升高明显(P<0.05);随再灌注时间的延长,MDA含量逐渐升高,尤为再灌注2、6、24h(P<0.01);缺血10min组与缺血30min组相比,后组明显高于前组,尤为再灌注6h(P<0.01)。结论宫内缺血无再灌注时即有自由基损伤的存在;自由基损伤可能是宫内缺血再灌注肾损伤的发病机制之一。

Effect of intra-uterine actute ischemia/reperfusion on superoxide dismutase and malondialdehyde in fetus rat kidney

Objective To study the changes of superoxide dismutase and malondialdehyde in fetus rat kidney during actute ischemia/reperfusion injury and to explore pathophysiologic mechanism of renal ischemia/reperfusion injury in fetal rats.Methods The pregnant 21 days Wistar rats were anesthetized intraabdominaly.After two-horn uterus and vessels supplying uterus and ovary were exposed.arterial clamp occluded one side of vessels. Another side was regarded as sham operation group. The measured time after ischemia was 10min and 30min. The measured time afte reperfusion time was 30min. 2hr.6hr and 24hr. The contents of superoxide dismutase (SOD) and malondialdehyde (MDA) in both ischemia and reperfusion period were detected.Results (1)The activity of SOD weakened gradually with the extension of ischemia period. especially in 30minutes' ischemia group. It decreased progressively during reperfusion and reach minimum after 6hours' reperfusion.Then it began to increased.There was no difference between sham operation group and observed group in reperfused 24 hours.These changes showed significance in reperfusion group after 30 minutes ischemia.(2)The contents of MDA increased little by little in ischemia and reperfusion period especially at 30 minutes' ischemia group.Conclusions When there is simply ischemia.the activity of decreased SOD and increased MDA indicated that free radicals could be produced in this stage. After reperfusion.changes of SOD and MDA prove that free radicals injury may be one of mechanisms of renal injury.