苗药观音草总皂苷水提取工艺的研究 |
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引用本文: | 刘亮,丁丽娜,万路平,陈东林,陈荣华,吴永秀. 苗药观音草总皂苷水提取工艺的研究[J]. 承德医学院学报, 2014, 0(6): 470-471 |
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作者姓名: | 刘亮 丁丽娜 万路平 陈东林 陈荣华 吴永秀 |
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作者单位: | 1. 遵义医药高等专科学校,贵州遵义,563002 2. 遵义市果蔬工作站 |
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基金项目: | 遵义市科技局、遵义医药高等专科学校联合科技计划项目,遵义市红花岗区科学技术项目 |
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摘 要: | 目的:优化观音草总皂苷的水提取工艺。方法:采用正交试验法,以总皂苷的含量为考察指标。结果:薯蓣皂苷的线性范围是4-24μg/ml,r=0.9995。各因素对总皂苷得率的影响顺序为提取次数>液料比(ml/g)>提取温度>提取时间,其中液料比和提取次数对总皂苷得率具有显著影响(P<0.05)。结论:观音草中总皂苷的最佳提取工艺为:提取温度70℃,提取时间30min,液料比12:1(ml/g),提取次数3次。
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关 键 词: | 观音草 总皂苷 提取工艺 |
STUDY ON WATER EXTRACTION TECHNIQUE OF TOTAL SAPONINS INREINECKIA CARNEA (ANDR.) KUNTH |
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Affiliation: | LIU Liang, DING Li-na, WAN Lu-ping, et al (Zunyi Medical and Pharmaceutical College, Guizhou Zunyi 563002, China) |
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Abstract: | Objective: To optimize the water extraction technique of total saponins inReineckia carnea (Andr.) Kunth. Methods:The orthogonal optimization experiment was used in this study with the total saponins content inReineckia carnea (Andr.) Kunth as the index.Results: The linear range of dioscin was 4-24μg/ml (r=0.9995). The sequence of every factor for rate of the total saponins inReineckia carnea (Andr.) Kunth was extraction times〉liquid-to-solid ratio (ml/g)〉extraction temperature〉extraction duration. Among them, the liquid-to-solid ratio and extraction times had significant influence on total saponins yield.Conclusions:The best extraction technique of total saponins inReineckia carnea (Andr.) Kunthis extraction temperature 70℃, extraction duration 30 minutes,liquid-to-solid ratio 12:1 (ml/g), extraction 3 times. |
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Keywords: | Reineckia carnea (Andr.) Kunth Total saponins Extraction technique |
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