| HPLC-DAD-MS法定量分析黄芩中3种黄酮类化合物 |
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| 引用本文： | 徐杨璐,赵胜男,申川川,李守拙.HPLC-DAD-MS法定量分析黄芩中3种黄酮类化合物[J].承德医学院学报,2014(6):461-463. |
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| 作者姓名： | 徐杨璐 赵胜男 申川川 李守拙 |
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| 作者单位： | 河北省中药研究与开发重点实验室，承德医学院中药研究所，河北承德 067000 |
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| 摘 要： | 目的：分析比较高效液相色谱-二极管阵列检测器-质谱（HPLC-DAD-MS）法和高效液相色谱-二极管阵列检测器（HPLC-DAD）法检测黄芩中3种黄酮类化合物的结果。方法：分别采用HPLC-DAD-MS法和HPLC-DAD法检测黄芩中3种黄酮类化合物黄芩苷、汉黄芩苷、汉黄芩素的含量。结果：HPLC-DAD-MS法测得黄芩苷、汉黄芩苷、汉黄芩素的含量分别比HPLC-DAD法平均少6.61%、73.98%和10.90%，其中汉黄芩苷的含量相差较大。结论：与HPLC-DAD法相比，HPLC-DAD-MS法含量测定结果更准确，且操作简便、重现性好，可用于黄芩中黄芩苷、汉黄芩苷、汉黄芩素的含量测定。
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| 关 键 词： | 黄芩 高效液相色谱-二极管阵列检测器-质谱法 质量控制 |
ANALYSIS OF THREE FLAVONOIDS INSCUTELLARIA BAICALENSIS GEORGI BY HPLC-DAD-MS |
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| Institution: | XU Yang-lu, ZHAO Sheng-nan, SHEN Chuan-chuan, et al (Hebei Key Laboratory of Research and Exploitation of Traditional Chinese Medicine, Institute of Chinese Materia Medica, Chengde Medical College, Hebei Chengde 067000, China) |
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| Abstract: | Objective：To compare the determination of three lfavonoids inScutellaria baicalensis Georgi by high performance liquid chromatography with diode array detection and mass spectrometer （HPLC-DAD-MS） and high performance liquid chromatography with diode array detection （HPLC-DAD）.Methods： HPLC-DAD-MS and HPLC-DAD were respectively applied to determineBaicalin,Wogonoside and Wogonin inScutellaria baicalensis Georgi. Results：The content ofBaicalin, Wogonoside, Wogonin by HPLC-DAD-MS were 6.61%, 73.98% and 10.90% less than HPLC-DAD. Among them, the difference ofWogonoside content was fairly larger.Conclusions： Compared with HPLC-DAD, HPLC-DAD-MS is more accurate, simple and convenient. So HPLC-DAD-MS can be used for the determination of Baicalin, Wogonoside, WogonininScutellaria baicalensis Georgi. |
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| Keywords: | Scutellaria baicalensis Georgi HPLC-DAD-MS Quality control |
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