辛伐他汀对成骨细胞分化和成骨基因的影响

目的 探讨辛伐他汀对成骨细胞分化及成骨基因表达的影响.方法 选取成骨肉瘤细胞株 MG-63,采用不同浓度辛伐他汀（0.0625、0.125、0.25、0.5和 1.0 μmol/L）处理成骨细胞,ALP活性检测辛伐他汀对成骨细胞分化作用的影响;实时定量 RT-PCR和免疫印迹检测细胞成骨基因 mRNA的表达.结果 通过不同浓度辛伐他汀处理成骨细胞后,不同浓度辛伐他汀组与对照组比较,成骨细胞ALP 活性差异均有统计学意义 （ P〈0.05）,其中 0.25 μmol/L 浓度组作用对成骨细胞碱性磷酸酶活性的影响最为显著.采用 0.25 μmol/L辛伐他汀处理成骨细胞后,辛伐他汀组与对照组比较,成骨细胞骨钙蛋白、ALP、I型胶原 mRNA表达差异均有统计学意义 （ P〈0.05）.结论 辛伐他汀可能通过上调成骨基因mRNA的表达水平来促进其分化,这为他汀类药物治疗骨质疏松症提供新的干预靶点.

Effects of simvastatin on osteoblast differentiation and osteogenic genes expression

Objective To explore the effect of simvastatin on osteobiast differentiation and osteogenic gene expression. Methods To select osteosareoma cell line MC,-63, using different concentrations of simvastatin （0.0625,0.125, 0.25,0.5 and 1.0 μmol/L） treated osteoblasts, ALP activity detection simvastatin on osteoblast differentiation of affected. Results Through the treatment of different concentrations for osteoblasts, different concentrations of simvastatin group compared with the control group, ALP activity of osteoblasts differences were statistically significant （P〈0.05）, using 0.25 pano]/L simvastafin treated osteoblasts, the simvastatin group and control group comparison, osteoblasts osteocalcin, ALP, I collagen mRNA expression differences were statistically significant （P〈0.05）. Conclusion Simvastatin by upregadating the expression of osteogenic gene mRNA levels to promotes their differentiation, which provides new targets in the prevention of osteogenic for statins.