GDNF对脊髓前角运动神经元的保护作用

目的：证实胶质源性神经营养因子（glial cell line-derived neurotrophic factor,GDNF)对脊髓前角运动神经元有保护作用。方法：切断SD大鼠一侧坐骨神经建立脊髓前角运动神经元损伤模型。借助单盲端硅胶管系统在损伤神经局部给予GDNF，术后不同时间分别取L5脊髓切片，利用酶组织化学染色方法显示胆碱酯酶（CHE）和酸性磷酸酶（ACP）活性并进行图像分析。结果：坐骨神经切断可导致腰段脊髓前角运动神经元明显损害，表现为CHE活性降低。ACP活性升高；应用GDNF可显著改善上述酶学变化。结论：GDNF对损伤的脊髓前角运动神经元有保护作用。

Protective Effect of Glial Cell Line-derived Neurotrophic Factor on Spinal Front Corner Motor Neurons

Objective: To investigate the protective effect of glial cell line derived neurotrophic factor(GDNF) on spinal anterior corner motor neurons. Methods: The sciatic nerve was sectioned unilaterally and GDNF was suppiled locally to the proximal nerve stump using a single blind silicone tube. The specimen of L 5 spinal cord were taken at different time postoperatively and enzyme histochemical technique of ACP and CHE were conducted, image analysis of section using computer was applied. Results: Sciatic nerve section lead to obvious injury of the spinal motor neurons which showed a decrease in CHE activity and increase in ACP activity. Application of GDNF significantly improve the above mentioned enzymatic activity changes. Conclusion: GDNF plays a role in protecting the spinal anterior corner motor neuron against injury.