糖尿病大鼠缺血再灌注脑组织脑源性神经生长因子和碱性成纤维细胞生长因子的表达及意义

目的探讨糖尿病性高血糖大鼠脑缺血再灌注后脑组织脑源性神经生长因子(brain-drived neurotropic factor,BDNF)和碱性成纤维细胞生长因子(basic fibroblast growth factor,bFGF)的表达及意义。方法41只Wistar大鼠随机分为正常对照组、假手术组、正常血糖脑缺血再灌注组(NIR)和糖尿病性高血糖脑缺血再灌注组(DIR),用链脲佐菌素腹腔注射和线栓法分别诱发大鼠糖尿病和复制大脑中动脉缺血再灌注模型,应用免疫组化法检测再灌注6h和24h脑组织BDNF和bFGF蛋白表达。结果大鼠脑缺血再灌注后BDNF主要在梗死区表达,bFGF主要在梗死灶周边区表达,NIR组BDNF和bFGF的表达在再灌注6h组和24h组均较假手术组和正常对照组明显增加(P<0.05);DIR组BDNF和bFGF的表达在再灌注6h组和24h组较NIR组明显下降(P<0.05)。结论大鼠脑缺血再灌注后脑组织BDNF和bFGF表达增加可能是内源性保护机制;糖尿病性高血糖大鼠脑缺血再灌注后脑组织BDNF和bFGF表达降低可能是糖尿病加重缺血损伤的机制之一。

The expression of BDNF, bFGF and its significance in hyperglycemic rats with cerebral ischemic-reperfusion

Objective To observe the expression of BDNF(brain-drived neurotropic factor),bFGF(basic fibroblast growth factor)and its significance in hyperglycemic rats with cerebral ischemic-reperfusion.Methods 41 Wistar rats were randomly divided into four groups:hyper glycemia(n=12),normoglycemia(n=12),sham operation(n=12)and control group(n=5).The rats in former three groups were respectively divided into two subsets:reperfusion for 6h and 24 h(n=6).Hyperglycemia model was made by injection of streptozocin(STZ)through abdomen in Wistar rats.The model of cerebral ischemia reperfusion was developed by thread embolism method.The expresstion of BDNF and bFGF were detected by immunohistochemistry.Results The expression of BDNF and bFGF in a rat with cerebral ischemia reperfusion was mainly observed around ischemia.Compared with sham operation and control group,the expression of BDNF and bFGF increased evidently in NIR groups.But compared with NIR groups,the expression of BDNF and bFGF decreased in DIR groups.Conclusion Increased expression of BDNF and bFGF may play an important role in neuroprotection in a rat with cerebral ischemia reperfusion.Down-regulated expression of BDNF and bFGF may be aggravated with cerebral ischemic reperfusion injury in diabetes mellitus rats.