Comparison of blood component preparation methods from whole blood bags based on buffy coat extraction期刊界 All Journals 搜尽天下杂志传播学术成果专业期刊搜索期刊信息化学术搜索

Comparison of blood component preparation methods from whole blood bags based on buffy coat extraction

Affiliation:	1. Cancer Genetics Research Unit, Reza Radiation Oncology Center, Mashhad, Iran;2. Department of Biology, Faculty of Science, University of Zabol, Zabol, Iran;3. International Consultancy in Blood Components Quality/ Safety Improvement, Audit/ Inspection and DDR Strategies, London, United Kingdom;1. Department of Laboratory Medicine, University of Ulsan College of Medicine and Asan Medical Center, Seoul, South Korea;2. Department of Laboratory Medicine, Kangdong Sacred Heart Hospital, Seoul, South Korea;3. Department of Laboratory Medicine, Seoul National University Hospital, Seoul, South Korea;1. Department of Chemistry, Faculty of Science, Fukuoka University, Jonan-ku, Fukuoka 814-0180, Japan;2. Research Institute of Biosciences, School of Veterinary Medicine, Azabu University, Sagamihara, Kanagawa 252-5201, Japan

Abstract:	We compared the data of our quality control laboratory of the blood components according to the blood component preparation method that we used. We prepared blood components from top and top whole blood (WB) bags and manual pooling of buffy coats (BCs) (method I) or from top and bottom WB bags and automated pooling of BCs with OrbiSac (method II). Pooled platelet concentrates (PC) obtained with method II had higher platelet content when compared with pooled PCs obtained with method I (3.5 ± 0.7 × 10¹¹ vs. 2.6 ± 0.8 × 10¹¹; p < 0.001). The hemoglobin content in the RBCs obtained with method I was higher when compared with method II (55 ± 7 g vs. 52.5 ± 6.6 g; p < 0.001).

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