| 重组痘苗病毒为载体表达脊髓灰质炎病毒（Ⅰ型）及甲型肝炎病毒双价抗原 |
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| 引用本文： | 刘金辉,苏晔,戴长柏,郭仁. 重组痘苗病毒为载体表达脊髓灰质炎病毒（Ⅰ型）及甲型肝炎病毒双价抗原[J]. 中国医学科学院学报, 1996, 0(3) |
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| 作者姓名： | 刘金辉 苏晔 戴长柏 郭仁 |
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| 作者单位： | 中国医学科学院，中国协和医科大学医学生物学研究所 |
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| 摘 要： | 本文报道含有脊髓灰质炎病毒Ⅰ型中和抗原位点及甲型肝炎病毒中和抗原位点重组痘苗病毒的构建，用重组痘苗病毒感染１４３ＴＫ－细胞后成功地表达了脊髓灰质炎病毒Ⅰ型和甲型肝炎病毒的抗原。免疫荧光显示，两种抗原的荧光颗粒均分布在胞浆内。Ｗｅｓｔｅｒｎｂｌｏｔ证实，重组痘苗病毒所表达的脊髓灰质炎病毒Ⅰ型抗原和甲型肝炎病毒抗原为一融合蛋白。用此重组痘苗病毒免疫动物，可诱导脊髓灰质炎病毒Ⅰ型和甲型肝炎病毒的中和抗体产生。
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| 关 键 词： | 痘苗病毒；脊髓灰质炎病毒；甲型肝炎病毒 |
Expressing Poliovirus (Type I)and Hepatitis A Virus Antigens by Recombinant Vaccinia Virus |
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| Liu Jinhui,Su Ye,Dai Changbai,Guo Ren. Expressing Poliovirus (Type I)and Hepatitis A Virus Antigens by Recombinant Vaccinia Virus[J]. Acta Academiae Medicinae Sinicae, 1996, 0(3) |
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| Authors: | Liu Jinhui Su Ye Dai Changbai Guo Ren |
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| Abstract: | A recombinant vaccinia virus expressing PV-I and HAV antigens was constructed.Dot blot and Southern blot confirmed that the recombinant virus genome contained PV-I cDNA and HAV cDNA chimeric fragment.The result of IF indicated that PV-I antigen and HAV antigen were successfully expressed in the mammalian cells infected with the recombinant virus.The expressed PV-I and HAV antigen showed one band which had both PV-I and HAV antigenicities in Western blot.The band was about 120000(MW).When guinea pigs were immunized with the recombinant virus,PV-I and HAV neutralizing antibodies were induced. The result indicated that the recombinant virus constructed here had bi-valence of PV-I and HAV antigen as well as PV-I and HAV immunogenicities. |
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| Keywords: | vaccinia virus poliovirus hepatitis A virus |
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