雌二醇对巨噬细胞的激活作用

目的: 研究17β-雌二醇对腹腔巨噬细胞的影响,试图分析其在子宫内膜异位症病理过程中的作用及作为药物研究靶点的可能性。方法:扫描电镜观察巨噬细胞表面形态;Griess's试剂检测NO的含量;TNF-α采用L929细胞为靶细胞的细胞毒MTT法测定;激光扫描共聚焦显微镜检测胞浆内钙离子浓度[Ca²⁺]i。结果:17β-雌二醇:(1)使细胞表面微绒毛增多、增粗、密集,细胞表面有较多颗粒状隆起,皱褶增多;(2)促进巨噬细胞释放NO;(3)诱导巨噬细胞产生TNF-α;(4)100 nmol/L 17β-雌二醇使巨噬细胞的[Ca²⁺]i 明显提高(提高39.8%)。结论:一定浓度的17β-雌二醇激活巨噬细胞,是内异症的病理环节之一。

Effect of 17β-estradiol on macrophages

AIM: To analyze the Effect 17β-estradiol on activated macrophages and possibility of being a target of screening drugs for endometriosis. METHODS: Using scanning electron microscope, the surface of macrophages was observed. Nitric oxide (NO) in supernatant was determined by Griess's reagent. TNF-α was measured by MTT via L929 cells. Using Fluo-3/AM,[Ca²⁺]i was measured by laser scanning confocal microscopy (LSCM).RESULTS:(1)Increased size,extension of more microvilli,expression of retraction fibers and elaboration of membrane ruffles were detected in 17β-estradiol treated macrophages.(2)17β-estradiol induced NO release from peritoneal macrophages.(3)The more TNF-αwas made by macrophages under the induction of 1β7-estradiol.(4)In-tracellular Ca₂₊ elevated 39.8%in peritoneal macrophages after 17β-estradiol(100 nmol/L)treatment. CONCLUSIONS: Macrophages were activated by 17β-estradiol at a certain concentration. Activated macrophages by 17β-estradiol may participate in endometriosis and may be a target of screening drugs for endometriosis.