miR-133在胃癌组织中的表达及对胃癌细胞恶性行为的影响

目的 探讨microRNA-133（miR-133）在胃癌组织及细胞系中的表达情况，并探讨其对胃癌细胞凋亡和侵袭的影响。方法 采用实时定量PCR（qPCR）检测64例胃癌组织及对应癌旁组织中的miR-133水平，分析miR-133表达与临床病理参数（性别、年龄、肿瘤位置、临床分期、分化程度、浸润深度及淋巴结转移）的关系，并检测其在胃癌细胞株AGS、SGC-7901、MKN-1、MKN-45、MGC-803和BGC-823及正常胃黏膜细胞GES-1细胞的表达情况，同时选取miR-133水平最低的胃癌细胞并转染过表达miR-133的真核重组质粒pCDNA3.1+miR-133，转染后分别采用流式细胞仪PI/Annexin V双染法及Transwell法检测miR-133对细胞凋亡和侵袭能力的影响。结果 胃癌组织的miR-133相对表达量为0.347±0.024，低于癌旁组织（P＜0.05），且其表达与临床分期、分化程度、浸润深度及淋巴结转移有关均有关（P＜0.05）；与GES-1细胞相比（其miR-133表达水平设为1.00），胃癌细胞的miR-133水平均较低（P＜0.05），且MKN-45的表达水平最低；与对照组和空转染组相比，过表达组转染48、96 h后的细胞凋亡水平均升高，且穿膜细胞数均降低（P＜0.05）。结论miR-133在胃癌组织和细胞中均为低表达，上调miR-133水平可抑制胃癌细胞的侵袭并诱导凋亡。

Expression of miR-133 in gastric cancer tissues and its effect on malignant behavior of human gastric cancer cells

Objective To investigate the expression of microRNA-133（miR-133） in gastric cancer tissues and cell lines as well as its effect on apoptosis and invasion of gastric cancer cells. Methods The real-time quantitative PCR （qPCR） was used to detect the miR-133 level in 64 cases of gastric cancer tissues and corresponding adjacent normal tissues. Then， we analyzed the relationship between miR-133 expression and clinical pathological parameters（age， gender， tumor location， clinical stage， differentiation degree， invasion depth and lymph node metastasis）. The expression of miR-133 was detected in gastric cancer cell line AGS， SGC-7901， MKN-1， MKN-45， MGC-803， BGC-823 and GES-1， and the normal gastric mucosa cell line， GES-1， was used as the control. The gastric cancer cell with the lowest level of miR-133 was chosen for the transfection with the recombinant plasmid pCDNA3.1+miR-133. PI/Annexin V double staining and Transwell assay were used to detect the effect of miR-133 on apoptosis and invasion of cells. Results The relative expression of miR-133 in gastric carcinoma tissues was 0.347±0.024， lower than that of the adjacent tissues （P＜0.05）. Its expression was related to clinical stage， differentiation， depth of invasion and lymph node metastasis （P＜0.05）. Compared with the GES-1 cells， the miR-133 levels of gastric cancer cells were lower （P＜0.05）， and the expression level of MKN-45 was lowest. Compared with other two groups at 48 and 96 h after transfection， the apoptosis level was higher but the number of the cells was decreased in the overexpression group （P＜0.05）. Conclusion miR-133 was lowly expressed in gastric cancer tissues and cells， and upregulation of miR-133 can inhibit the invasion and apoptosis of gastric cancer cells.