谷氨酰胺对短肠综合征大鼠肝脏及结肠形态的作用

目的：观察谷氨酰胺对短肠综合征大鼠肝脏和结肠形态的影响。方法：23只雄性大鼠切除80％小肠，随机分3组：饮食组8只术后自由进食，全胃肠外营养（TPN）组8只输入TPN标准液，谷氨酰胺（Gln)组7只，输TPN＋Gln，正常大鼠8只，作为对照组。术后第7天，取门静脉血测定肝功能，肝脏和结肠进行组织学检查，结果：饮食组、正常组和Gln组血ALb明显高于TPN组，Gln组血TP明显高于TPN组，光镜下Gln组（2／7）和TPN组（3／8）肝脏标本呈脂肪变性改变，两无显差别，饮食组结肠粘膜厚度明显大于正常组，Gln组结肠粘膜厚度明显大于TPN组。结论：Gln促使结肠粘膜增生，有利于维持血TP和ALb水平，但未能减轻肝脂肪变性。

The effect of Glutamine on shape of liver and colon in rats with short bowel syndrome.

Objective　The present study investigated the effect of Glutamine on shape of liver and colon in rats with short bowel syndrome.　Methods　23 male Sprague-Dawley rats underwent a 80% small bowel resection,randomly divided into three groups:food group(n=8) rats was fed with rat chow and water ad libitum after operation;total parenteral nutrition(TPN) group(n=8) was infused with standard TPN;glutamine(Gln) group(n=7) was infused with Gln-supplyed TPN; eight normal male rats served as normal control group.On seventh day after operation,hepatic function was determined,liver and colon was harvested for histological observation (include optic microscopy and electron microscopy).　Results　ALb in food group、control group and Gln group was significantly higher than that in TPN group,TP in Gln group was significantly higher than in TPN group, There was hepatic steatosis change in Gln group(2/7) and TPN group(3/8) in optic microscopy,without statistical significant difference between Gln group and TPN group.Colonic mucosal thickness in food group was significantly bigger than that of control group, Colonic mucosal thickness in Gln group was significantly bigger than that in TPN group。　Conclusions　Gln promoted colonic mucosal hypertrophy.Gln dould not decrease hepatic steatosis in the present experimental condition,Gln was favorable to maintain TP and ALb level of blood.