1,6-二磷酸果糖对冻融移植小鼠卵巢组织的影响

目的:在冷冻液中增加能量底物1,6二磷酸果糖(Fructose-1,6-bisphosphate,FDP),观察FDP是否对冻存卵巢有保护作用.将9～12周龄小鼠的卵巢取出,在含(FDP组)或不含FDP(LN2V组)的冷冻液中平衡后快速深低温冻存,解冻后行自体双肾被膜下移植,观察动情周期、移植21 d后取移植物固定行H...

Effect of fructose-1,6- bisphosphate on frozen-thawed and transplanted ovaries in mice

Objective：To observe the effects of freezing solution containing fructose-1,6-bisphosphate（FDP） on viability of frozen-thawed ovarian tissue in mice.Methods：Mouse ovaries（2×1×1mm3 in size） were frozen with rapid freezing method,using freezing solution containing（FDP group） or not containing FDP（LN2V group）.After being thawed,autotransplantations were performed and two pieces of ovarian tissue slices were respectively implanted to each mouse under the kidney capsule of both sides.The survival of ovarian grafts,histological structure,follicular density and serum E_2 level in each recipient were detected 21 days after transplantation.The viability of frozen-thawed ovarian tissue was compared between the two groups.Results：The number of follicles with normal morphology in FDP group was 3.6±0.7/HP,and the serum E_2 level was 8.5±0.9 pg/ml,which were both significantly higher than that of the LN_2V group（P〈0.05）.Conclusions：The freezing solution containing FDP could increase the number of follicles,level the surm E_2,as well as promote the cryopreservation effects of ovarian tissues.