脑血管病患者血清内源性氧化剂和抗氧化剂水平变化的研究

目的 :观察脑血管病 (CVD)患者的血清内源性氧化剂及抗氧化剂水平变化及其临床意义。方法 :采用生物化学方法 ,测定 4 9例脑出血 (CH)、6 5例脑梗死 (CI)以及 35例神经系统其它疾病患者和 34名健康人(对照组 )的血清一氧化氮 (NO)、一氧化氮合酶 (NOS)、丙二醛 (MDA )、维生素 E(Vit E)、维生素 C(Vit C)、超氧化物歧化酶 (SOD)水平。结果 :CH组和 CI组血清 NO、MDA含量和 NOS活性与另两组相比均显著增高 (P<0 .0 5 ,P<0 .0 1) ,于发病 3d内升高显著 ;而血清 Vit E、Vit C含量和 SOD活性均低于两个对照组 (P<0 .0 5或P<0 .0 1)。结论 :NO、NOS参与了 CH和 CI脑组织损伤的病理过程 ,检测血清 NO、Vit E、Vit C、MDA含量及NOS、SOD活性有助于判断 CH和 CI患者病变程度和病情进展。

Study on the changes in endogenous oxidation agents and levels of anti-oxidation agents in patients with cerebral vascular disease

Objective:To investigate serum levels of endogenous oxidation agents,antioxidation agents and clinical significance in the patients with cerebral vascular disease (CVD).Methods:Using biochemical methods,the levels of serum nitric oxide (NO),nitric oxide synthase (NOS),malondialdehyde (MDA) and antioxidants vitamin E (VitE),vitamin C (VitC),superoxide dismutase (SOD) in 49 patients with cerebral hemorrhage (CH),65 patients with cerebral infarction(CI) and 35 patients with other nervous system diseases and 34 healthy controls were determined.Results:In CH and CI groups,the levels of serum NO and MDA and the activity of serum NOS were significantly higher than that of the two other groups ( P <0.05 or P <0.01).On the other hand,the patients with CH and CI had lower VitE,VitC levels and SOD activity than that of the two control groups ( P <0.05 or P <0.01).Conclusion:These findings suggest NO and NOS plays an important role in pathogenesis of cerebral damage after CH and CI.Determination of the concentrations of NO,VitE,VitC,MDA level,and NOS and SOD activity in serum can also help judge the seriousness and the course of the disease.