再生障碍性贫血患者对环胞菌素A治疗的反应与骨髓上清TNF- α,IFN-γ水平的关系

目的了解再生障碍性贫血(再障)患者骨髓上清TNF-α,IFN-γ的水平,探讨其与免疫抑制剂环胞菌素A(CsA)治疗反应的关系.方法采用酶联免疫(ELISA法)对24例再障患者及20名正常人骨髓上清TNF-α,IFN-γ进行检测,所有再障患者给予CsA治疗至少3个月.结果再障病人骨髓上清TNF-α,IFN-γ水平与正常对照组比较均有明显统计学差异(P＜0.05～0.01),TNF-α,IFN-γ水平高者对CsA治疗的反应好.结论再障患者骨髓上清TNF-α,IFN-γ水平较正常对照组均有不同程度的增高,对CsA治疗的反应可能与骨髓上清TNF-α,IFN-γ水平有关.

Relationship between response of aplastic anemia patients to cyclosporin therapy and levels of tumour necrosis factor-α, interferon-γof bone marrow plasma from patients with aplastic anemia

Objective] To detect the levels of tumour necrosis factor-α(TNF-α), Interferon-γ(IFN-γ) of bone marrow plasma (BMP) from the patients with aplastic anemia and explore the relationship between the levels of those cytokines and the effects of cyclosporin A (CsA) therapy. Methods] Endogenous TNF-α, IFN-γ in the BMP were measured in 24 aplastic anemia patients and 20 normal control by enzyme-linked immunosorbent assay (ELISA)methods. All patients were administered with the CsA at least three month. Results] The levels of TNF-α, IFN-γin the BMP of the aplastic anemia patients were significantly increased than in the normal control (P ＜0.05～0.01),the patients to follow the high levels of TNF-α, IFN-γ have a good response to CsA therapy. Conclusion] The levels of endogenous TNF-α, IFN-γ in the BMP of aplastic anemia were high, the effects of CsA therapy may be related with the levels of TNF-α, IFN-γin the BMP.