| 高效液相色谱法测定去氧氟尿苷含量及其有关物质 |
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| 引用本文： | 郝万红,孙红梅.高效液相色谱法测定去氧氟尿苷含量及其有关物质[J].中国药业,2007,16(1):29-30. |
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| 作者姓名： | 郝万红 孙红梅 |
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| 作者单位： | 1. 山东万杰高科技股份有限公司制药厂,山东,淄博,255213
2. 山东淄博万杰医院,山东,淄博,255213 |
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| 摘 要： | 目的 用高效液相色谱法（HPLC法）测定去氧氟尿苷的含量及其有关物质。方法 采用Diamonsil C—粒（250mm×4．6mm，5μm），流动相为乙腈-水（1：1），检测波长为269nm，流速为0．8mL／min。结果 去氧氟尿苷质量浓度在10．0～200．4μg／mL范围内与峰面积线性关系良好，r：0．9999（n：5），平均回收率为100．2％，RSD=0．14％。结论 HPLC法简便、准确，专属性好，灵敏度高，可用于去氧氟尿苷含量及有关物质的测定。
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| 关 键 词： | 去氧氟尿苷 含量测定 有关物质 高效液相色谱法 |
| 文章编号： | 1006-4931（2007）01-0029-02 |
| 收稿时间： | 2006-02-17 |
| 修稿时间： | 2006-05-13 |
Determination of the Content of Doxifluridine and Its Related Substances by HPLC |
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| Hao Wanhong,Sun Hongmei.Determination of the Content of Doxifluridine and Its Related Substances by HPLC[J].China Pharmaceuticals,2007,16(1):29-30. |
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| Authors: | Hao Wanhong Sun Hongmei |
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| Institution: | Hao Wanhong, Sun Hongmei( 1. Pharmaceutical Factory, Shandong Wanfie High Tech. Stock Co., Ltd., Zibo, Shandong, China 255213; 2. Wanjie Hospital of Zibo, Shandong Province, Zibo, Shandong, China 255213) |
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| Abstract: | Objective To establish HPLC method for determination of doxifluridine content and its related substances. Methods The column was Diamonsil-Cts column （250ram ×4.6mm,5μn）.The mobile phase was acetonitrile-water （1 ： 1）.The detection wavelength was 269 nm. The flow rate was 0. 8 mL/min. Results There was a good linear relationship with the range of 10.0- 200.0 p,g/mL for determination of the doxifluridine＇s content, r=0.999 9（n=5）.The average recovery rate was 100. 2%, RSD=0. 14%. Conclusion The method is simple, accurate, sensitive and can be used to determine the content and the related substances of doxifluridine. |
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| Keywords: | doxifluridine content determination related substances HPLC |
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