血管紧张素转换酶基因多态性与中国汉族迟发性阿尔茨海默病的关系

目的：探讨血管紧张素转换酶（angiotensin I converting enzyme，ACE）基因插入（I）/缺失（D）多态性与阿尔茨海默病（Alzheimer disease，AD）的关系以及高血压对这种关系的影响。方法：采取病例-对照研究的方法，对象为96个符合精神疾病诊断和统计手册第4版（DSM-IV）诊断的AD病例和96名来自同一地区的性别、年龄匹配的非AD对照，用聚合酶链反应扩增，扩增产物经琼脂糖电泳溴化乙锭染色，进行多态性检测。结果：在病例和对照之间ACE基因型和等位基因分布差异有显著性，同样在有高血压的AD患者和对照之间基因型分布的差异有显著性；但血压正常的AD患者和对照之间基因型分布差异无显著性。结论：ACE基因的多态性与AD发病风险有关，但II基因型作为危险因素仅限于高血压的AD患者。

The association between angiotensin Ⅰ converting enzyme gene polymorphism and Chinese late onset Alzheimer disease

OBJECTIVE: To investigate the relationship between angiotensin I converting enzyme gene insertion/deletion polymorphism and Alzheimer disease (AD), as well as the effect of hypertension on the relationship. METHODS: This case-control study, included 96 AD patients meeting the DSM-IV diagnosis, and 96 subjects as controls coming from the same area and in the same environmental condition. Using the polymerase chain reaction (PCR) amplified the DNA segments, and the PCR products were identified by 2% agarose gel and visualized by ethidium bromide staining. RESULTS: There was significant difference between AD patients and controls in ACE genotypes and alleles distribution, as well as between AD patients with high blood pressure and controls with high blood pressure. But between normotensive AD patients and normotensive controls, there was no significant difference in ACE genotypes distribution (P>0.05). CONCLUSION: ACE genotypes associated with the risk of AD, but II genotype as risk genetic factor only restricted in subjects with high blood pressure.