雌激素对去势雌兔动脉粥样硬化形成的抑制作用

目的 研究雌激素对去势雌兔动脉粥样硬化形成的影响.方法 24只3月龄新西兰雌兔随机分为正常组对照、假手术组、去势对照组（卵巢切除）、雌激素组（卵巢切除并肌内注射苯甲酸雌二醇）,除正常对照组外均于手术2周后给予高脂饮食,雌激素组同时肌内注射给药,连续12周.12周末取主动脉行组织形态学观察,并在扫描电子显微镜下行病理学检查,应用免疫组织化学方法检测主动脉血管细胞黏附分子-1的蛋白表达.结果 12周末,与去势对照组比较,雌激素可显著减轻主动脉的动脉粥样硬化病理损害,抑制主动脉血管细胞黏附分子-1的蛋白表达.结论 雌激素可能通过抑制血管内皮细胞黏附分子的表达从而阻止动脉粥样硬化的发生发展.

Inhibitory effects of estrogen on atherosclerosis in ovariectomized rabbits

Objective To study the effects of estrogen on atherosclerosis in ovariectomized rabbits.Methods Twenty-four three-month-old female New Zealand white rabbits were randomly divided into four groups(n=6 each): the normal,the sham operation,the control(ovariectomized),and the treated(ovariectomized and treated with benzoestrofol).All of the rabbits,except the normal ones,were put on high-cholesterol diet two weeks after operation,and the treated group were injected the drug simultaneously.Twelve weeks later,the aortas were harvested for histomorphometry and scanning electron microscopy.The expression of VCAM-1(vascular cell adhesion molecule-1) was tested by immunohistochemistry.Results After 12 weeks of experiment,as compared with the control,the pathologic lesions in aortas were much less severe in the treated group,with the expression of VCAM-1 protein in aortas significantly reduced.Conclusion Estrogen could inhibit the VCAM-1 expression in aorta,contributing to the retardation of atherosclerosis in ovariectomized rabbits.