The effect of heterologous antisera and rheumatoid factor on the synthesis of DNA and protein by human peripheral lymphocytes |
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| Authors: | L J Holt N R Ling D R Stanworth |
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| Affiliation: | 1. Department of Radiology, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana;2. Spectra Health Imaging and Interventional Radiology, Kumasi, Ghana;3. Department of Molecular Medicine, Kwame Nkrumah University of Science and Technology, Kumasi, Ghana;4. Department of Radiology, Komfo Anokye Teaching Hospital, Kumasi, Ghana;5. Atasemanso Hospital Limited, Kumasi, Ghana;1. Università Cattolica del Sacro Cuore, Roma – Fondazione Gemelli - Dipartimento di Bioimmagini e Scienze Radiologiche, Rome, Italy;2. AORN “A.Cardarelli”, Napoli – UOSC Radiologia Vascolare ed Interventistica, Napoli, Italy;3. Università Cattolica del Sacro Cuore, Roma – Fondazione Gemelli, Rome, Italy;4. Policlinico Universitario Campus Biomedico – Roma, – UOC di Diagnostica per Immagini e Radiologia Interventistica, Rome, Italy |
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| Abstract: | Seven antisera prepared by immunizing rabbits with human leucocytes activated human lymphocytes in vitro to varying degrees.The relative activity of the antisera in terms of 14C-thymidine incorporation after 3 days of culture was the same whatever the source of the cultured lymphocytes.At low concentrations of some antisera considerable stimulation of DNA synthesis occurred even in the presence of complement. At high antiserum concentrations DNA synthesis was adversely affected in the presence, but not in the absence, of complement.Antisera against other human antigens, including immunoglobulins, were not active.Rheumatoid factor did not markedly augment the activity of leucocyte antibody.The level of DNA synthesis of sensitized lymphocytes in culture was markedly influenced by the source of the human serum present in the medium. |
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