| 仙台病毒载体对未成熟树突状细胞蛋白表达的影响 |
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| 引用本文： | 易发平,张平波,郭风劲,马永平,宋方洲.仙台病毒载体对未成熟树突状细胞蛋白表达的影响[J].第四军医大学学报,2007,28(3):214-216. |
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| 作者姓名： | 易发平 张平波 郭风劲 马永平 宋方洲 |
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| 作者单位： | 重庆医科大学生物化学与分子生物学教研室,重庆,400016;重庆市生物化学与分子药理学重点实验室,重庆,400016;临床检验诊断学省部共建教育部重点实验室,重庆,400016;日本九州大学遗传资源中心,福冈,日本,812-8581;重庆医科大学生物化学与分子生物学教研室,重庆,400016;重庆医科大学生物化学与分子生物学教研室,重庆,400016;重庆市生物化学与分子药理学重点实验室,重庆,400016;重庆医科大学生物化学与分子生物学教研室,重庆,400016;重庆市生物化学与分子药理学重点实验室;临床检验诊断学省部共建教育部重点实验室,重庆,400016 |
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| 基金项目： | 国家自然科学基金，重庆市高校优秀中青年骨干教师资助计划 |
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| 摘 要： | 目的:研究仙台病毒载体对未成熟树突状细胞(DC)蛋白质表达的影响. 方法:提取转染仙台病毒载体的树突状细胞和对照细胞的总蛋白,定量. 双向凝胶电泳分离蛋白质组分,胶体银染显色,PDQuest进行图像分析后选取差异点,胶内酶解后MALDI-TOF MS进行肽指纹图谱鉴定. 结果:图像分析结果显示,处理组2-DE图缺少了40个以上的蛋白点,对其中明显没有表达的5个蛋白点进行了鉴定. 结论:仙台病毒载体感染未成熟树突状细胞可引起蛋白表达的减少.
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| 关 键 词： | 仙台病毒载体 树突状细胞 蛋白质组 |
| 文章编号： | 1000-2790(2007)03-0214-03 |
| 修稿时间： | 2006-01-19 |
Protein changes in immature dendritic cells infected with sendai virus vector |
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| YI Fa-Ping,ZHANG Ping-Bo,GUO Feng-Jin,MA Yong-Ping,SONG Fang-Zhou.Protein changes in immature dendritic cells infected with sendai virus vector[J].Journal of the Fourth Military Medical University,2007,28(3):214-216. |
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| Authors: | YI Fa-Ping ZHANG Ping-Bo GUO Feng-Jin MA Yong-Ping SONG Fang-Zhou |
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| Institution: | 1.Department of Biochemistry and Molecular Biology, Chongqing University of Medical Sciences, 2.Chongqing Key Laboratory of Biochemistry and Molecular Pharmacology, 3.Key Laboratory of Clinical Laboratory Diagnostics of Ministry of Education, Chongqing University of Medical Sciences, Chongqing 400016, China, 4.Institute of Genetic Resources, Kyushu University, Fukuoka 812-8581, Japan |
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| Abstract: | AIM: To investigate the protein changes in immature dendritic cells (DC) infected with sendai virus vector. METHODS:The total cellular proteins were collected and determined in the immature DC infected with sendai virus vector and the control ones uninfected with it. They were separated using 2-dimensional gel electrophoresis (2-DE) and visualized by silver staining. Digital images were analyzed by PDQuest software. The differentially expressed protein spots were picked and digested in gel, followed by identification with peptide mass fingerprinting (PMF) using MALDI-TOF MS. RESULTS: More than 40 protein spots disappeared in 2-DE of the treated group. Among them 5 protein spots that were apparently not expressed were identified by PMF. CONCLUSION: Protein expression decreased in DC infected with sendai virus vector. |
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| Keywords: | sendai virus vector dendritic cell proteomic |
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