HPLC法同时测定芩连片中黄芩苷﹑黄芩素和汉黄芩素的含量

目的建立同时测定芩连片中黄芩苷、黄芩素和汉黄芩素含量的HPLC方法。方法采用Dia-monsil C18柱(200 mm×4.6 mm,5μm)分离,以甲醇-体积分数为0.4%的磷酸溶液为流动相梯度洗脱,流速为1.0 mL.min-1,检测波长为277 nm,柱温为35℃。结果黄芩苷、黄芩素及汉黄芩素的线性范围分别为36.8～368.0 mg.L-1(r=0.999 7)、1.75～17.5 mg.L-1(r=0.999 8)、1.50～15.0mg.L-1(r=0.999 9),黄芩苷、黄芩素、汉黄芩素的平均回收率分别为97.3%(RSD=1.8%)、101.1%(RSD=1.8%)、99.0%(RSD=2.0%)。结论 HPLC法可用于芩连片的质量控制。

Simultaneous determination of baicalin, baicalein and wogonin in Qinlian tablets by HPLC

Objective To establish a RP-HPLC method for determination of baicalin, baicalein and wogonin in Qinlian ( traditional Chinese medicines ) tablets. Methods The samples were separated on a Diamonsil ODS column (200 mm × 4.6 mm, 5 μm) through gradient delivery of a mixture of methanol - 0.4%(φ) phosphoric acid solution, the flow rate was 1.0 mL·min^-1. The detection wave-length was 277 nm and the temperature of column was 35℃. Results The linear ranges of baicalin, baicalein and wogonin were 36.8-368.0 mg·L^-1（r = 0.999 7）, 1.75-17.5 mg·L^-1 ( r = 0.999 8), and 1.50-15.0 mg·L^-1 (r = 0.999 9)，respectively. The average recoverys of the there components were 97.3 %（RSD = 1.8 %）,101.1 %（RSD = 1.8 %）,99.0 %（RSD = 2.0 %）, respectively. Conclusion The method is accurate and reproducible. It can be applied for the quality control of Qinlian tablets.