| 乙二胺四乙酸铵梯度法制备盐酸伊立替康脂质体 |
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| 引用本文： | 李哲,牟丽娜,王绍宁,杨强,邓意辉.乙二胺四乙酸铵梯度法制备盐酸伊立替康脂质体[J].沈阳药科大学学报,2011,28(12):933-937. |
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| 作者姓名： | 李哲 牟丽娜 王绍宁 杨强 邓意辉 |
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| 作者单位： | (1. 沈阳药科大学 药学院,辽宁 沈阳 110016;2. 辽源市中医院,吉林 辽源 136200;3.沈阳药科大学 制药工程学院,辽宁 沈阳 110016) |
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| 摘 要： | 目的制备盐酸伊立替康脂质体,考察包封率及粒径的影响因素。方法采用乙二胺四乙酸铵梯度法制备盐酸伊立替康脂质体,以阳离子交换树脂分离脂质体和游离药物,并以紫外分光光度法和激光粒度仪分别测定盐酸伊立替康脂质体的包封率和粒径;考察不同处方和制备工艺对脂质体包封率及粒径的影响。结果通过处方工艺优化,盐酸伊立替康脂质体包封率达到95.73%,粒径为112.8 nm。结论以乙二胺四乙酸铵梯度法制备的盐酸伊立替康脂质体包封率较高,方法可行。
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| 关 键 词： | 盐酸伊立替康 乙二胺四乙酸铵梯度法 脂质体 包封率 粒径 |
| 收稿时间： | 2011-5-29 |
Preparation of irinotecan hydrochloride liposomes by ammonium ethylenediaminetetraacetate gradient method |
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| LI Zhe,MU Li-na,WANG Shao-ning,YANG Qiang,DENG Yi-hui.Preparation of irinotecan hydrochloride liposomes by ammonium ethylenediaminetetraacetate gradient method[J].Journal of Shenyang Pharmaceutical University,2011,28(12):933-937. |
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| Authors: | LI Zhe MU Li-na WANG Shao-ning YANG Qiang DENG Yi-hui |
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| Institution: | (1. School of Pharmacy, Shenyang Pharmaceutical University, Shenyang 110016, China; 2. Lioayuan hospital of Traditional Chinese Medicine, Liaoyuan 136200, China; 3. School of Pharmaceutical Engineering, Shenyang Pharmaceutical University, Shenyang 110016, China) |
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| Abstract: | Objective To prepare irinotecan hydrochloride(CPT-11) liposomes, and to study the influence of factors on the entrapment efficiency and size. Methods CPT-11 was encapsulated in the liposomes by ammonium ethylenediaminetetraacetate(NH4EDTA) gradient method. Free CPT-11 and liposomes were separated by cation exchange resin. Ultraviolet spectrophotometry and laser particle analyzer was applied to determine the entrapment efficiency and the size of CPT-11 liposomes, respectively. The influence of various factors on the entrapment efficiency and size of CPT-11 liposomes were investigated. Results Through optimizing the formulations and preparation conditions, the CPT-11 liposomes were with high entrapment efficiency to 95.73% and the mean diameter to 112.8 nm. Conclusions The CPT-11 liposomes with high entrapment efficiency can be prepared by NH4EDTA gradient method, and the method was proved to be feasible. |
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| Keywords: | irinotecan hydrochloride ammonium ethylenediaminetetraacetate gradient method liposomes entrapment efficiency size" target="_blank">')">size |
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