清醒大鼠肺功能检测系统在全氟异丁烯吸入毒理研究中的应用

目的 考察在全氟异丁烯(PFIB)吸入毒理实验中引进清醒动物肺功能检测系统是否有助于PFIB中毒的诊断和治疗.方法 利用清醒动物肺功能检测系统,检测PFIB吸入中毒大鼠中毒前后不同时间的呼吸功能指标(吸气时间、呼气时间、呼吸频率、吸气流量峰值、呼气流量峰值、潮气量和呼气量).结果 Wistar大鼠给予PFIB 150 ...

Application of pulmonary function test system in the study of perfluoroisobutylene inhalation toxicology

Objective To measure if the pulmonary function test system of unrestrained animals could help diagnosis and treatment of perfluoroisobutylene (PFIB) inhalation toxicosis. Methods To record the change in pulmonary function parameters, such as inspiratory time (TI) , expiratory time (TE) , breathing frequency (F) , peak inspiratory flow (PIF) , peak expiratory flow (PEF) , tidal volume (VT) and expired volume (VE) , by unrestrained whole body plethysmography (EMKA Technologies, France) , before and after Wistar rats exposed to 150 mg/m³ PFIB for 8 min. Results The TI and TE decreased obviously at 4 h after PFIB exposure, and reduced to 129 ms and 215 ms at 8 h after PFIB exposure, respectively. The PIF, PEF and the breathing frequency increased significantly, on the contrary, the VT and VE decreased markedly at 8 h after PFIB exposure. Conclusion The pulmonary function test system can be handled easily, and can be used for measuring the changes in pulmonary parameters in the study of inhalation toxicology.