MS-302三道测量分析系统的应用及HI-6在离体膈神经-膈肌上抗梭曼的作用机制探讨

目的 探讨抗神经毒化合物HI-6在离体膈神经-膈肌上抗梭曼的作用机制.方法 采用MS-302三道测量分析系统,观察大鼠离体膈神经-膈肌的收缩功能,同时,测定膈肌乙酰胆碱酯酶(AChE)活性,观察药物对膈肌AChE活性的直接影响.结果 (1)采用MS-302三道测量分析系统对膈神经-膈肌标本强直收缩曲线下的面积进行测量,...

Application of MS-302 and effects of HI-6 on soman intoxication of isolated phrenic nerve diaphragm in rats

Objective To explore the mechanism underlying acetylcholinesterase （AChE） reactivation and recovery by HI-6 against soman toxication in isolated phrenic nerve and diaphragm in rats. Methods MS-302 was used to determine the function of phrenic nerve-diaphragmatic muscle. AChE activity assay was operated to assess the reactivation of soman induced inhibition of AChE by HI-6. Results （1）MS-302 was a real time record system that acculatedly determine the phrenic nerve-diaphragmatic muscle function. （2）HI-6 10 and 100 μmol/L had no effect on normal phrenic nerve-diaphragmatic muscle function, while HI-6 1 mmol/L had a positive inhibiting effect. When HI-6 10 μmol/L was administered preventively or therapeutically, no apparent protection was observed. While HI-6 100 μmol/L appeared protective effect. And an apparently protective effect was observed in 30 min at HI-6 1 mmol/L. （3）HI-6 adminstered prevently or therapeutically had no reactivating effect on AChE of rat diaphragms inhibited by soman. Conclusion It demonstrated that the recovery of phrenic diaphragm paralysis induced by soman is due to direct physiological antagonism of HI-6 instead of AChE reactivation.