乙型、丙型肝炎DNA疫苗单次联合接种后小鼠的免疫应答

目的：观察由编码ＨＢｓＡｇ与ＨＣＶ－ＣＥ２抗原的两种重组真核细胞表达质粒制备的ＤＮＡ疫苗联合接种ＢＡＬＢ／ｃ小鼠后,其施生行异性免疫应答的规律和相互影响。方法：应用上述２种ＤＮＡ疫苗单次联合免疫小鼠,动态观察血中特生抗体水平;并完成稳定转染,表达相应抗原的ＳＰ２／０骨髓瘤细胞的建株,采用ＣＴＬ杀伤活性体内诱生实验的方法建立观察ＤＮＡ疫苗免疫保护与治疗泊动物模型。结果：两种ＤＮＡ疫苗单次联合免疫小鼠

Study on the Immune Responses of Mice after Singly Inoculated with HBV and HCV Mixed DNA Vaccine

Aim: To study the immune response, of which the magnitude and strength after a mixed DNA vaccine of two kinds of eukaryotic expression plasmids encoded HBsAg and HCV-CE2 was singly inoculated BALB/c mice. Method: BALB/c mice singly inoculated with HBV and HCV mixed DNA vaccine, the immune response (special antibody and CTL) was compared between the immunization of this two kinds of plasmids separately and combined, along with the interaction of the two plasmids. The target cell line of stable transfection and expression of antigen was constructed, and the animal model of CTL test in vivo was first studied as a much advanced test compared with CTL test in vitro, which has a promising prospect both in preventive and therapeutic fields of studies. Results: The mixed DMA vaccine immunized mice induce immune response to both the plasmids. A weakly humoral response of mice to the expression products of S-gene and HCV-CE2 can be observed, meanwhile. the result of CTL test in vivo showed immuned mice has a prolonged life span after attacked by target cells (SP2/0 with expressing antigen), and there is a obvious value of the mixed vaccine , especially for HCV as the CTL response and memory cells were induced. Conclusion: This study created a simple, specific and effective method and gave some important information to further studies.