| 细胞培养中枯草杆菌污染的防控 |
| |
| 引用本文： | 李昆,于艳秋,金玉楠,杜莉莉,云伟. 细胞培养中枯草杆菌污染的防控[J]. 现代预防医学, 2007, 34(11): 2140-2141 |
| |
| 作者姓名： | 李昆 于艳秋 金玉楠 杜莉莉 云伟 |
| |
| 作者单位： | 中国医科大学病理生理教研室,沈阳,110001 |
| |
| 摘 要： | [目的]探讨青链霉素＋庆大霉素防控细胞培养中枯草杆菌污染的可行性。[方法]采用不同浓度青链霉素和庆大霉素配伍的方法，观察其抑制枯草杆菌的效果及对STO转基因小鼠滋养层细胞和D3系小鼠胚胎干细胞的生长情况的影响。[结果]700u／ml青链霉素和280u／ml庆大霉素联合应用的抑菌效果最佳，且对小鼠胚胎干细胞和小鼠成纤维细胞的生存率无明显影响。[结论]采用青链霉素和庆大霉素可以有效控制细胞培养中枯草杆菌的污染。
|
| 关 键 词： | 细胞培养 枯草杆菌 青链霉素 庆大霉素 |
| 文章编号： | 1003-8507（2007）11-2140-02 |
| 收稿时间： | 2006-07-20 |
| 修稿时间： | 2006-07-20 |
PREVENTING HAY BACILLUS CONTAMINATION IN CELL CULTURE |
| |
| LI Kun, YU Yan-qiu,Jin Yu-nan,et al.. PREVENTING HAY BACILLUS CONTAMINATION IN CELL CULTURE[J]. Modern Preventive Medicine, 2007, 34(11): 2140-2141 |
| |
| Authors: | LI Kun YU Yan-qiu Jin Yu-nan et al. |
| |
| Affiliation: | Department of Pathophysiology Basic medical science college China Medical University, Shenyang 110001, China |
| |
| Abstract: | [Objective] To study the feasibility of preventing hay bacillus contamination in cell culture by nrycillin and gentamyein. [ Methods] Myllicin and gentamycin were added into medium with different concentration, the survival of hay baciLlus, mice embryonic stem cells, and mice was observed. [Results] The combination of 700 u/ml nrylicin and 280 u/ml gentamycin was better than others in preventing hay bacillus, and it did not obviously affect the survival of mice embryonic stem cells, as well as fibroblast. [Conclusion] Mycillin and gentamycin can be used to prevent hay bacillus contamination in cell euhure. |
| |
| Keywords: | Cell culture Hay bacillus Myllicin gentamycin |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
|
