紫花地丁乙酸乙酯部位的化学成分研究

目的：对紫花地丁乙酸乙酯部位的化学成分进行研究。方法采用硅胶柱色谱、反相中压柱色谱和制备液相色谱等方法进行分离纯化，运用波谱数据并结合文献鉴定结构。结果分离得到了8个化合物，分别鉴定为槲皮素－3－O－β－D－葡萄糖－（1→4）－O－α－L－鼠李糖苷（1）、山奈酚－3－O－芸香糖苷（2）、山奈酚－3－O－β－D－葡萄糖－（1→2）－O－α－L－鼠李糖苷（3）、6，7－二羟基香豆素（4）、7－羟基－8－甲氧基香豆素（5）、6－羟基－香豆素－7－O－α－L－鼠李糖基－（1→6）－O－β－D－葡萄糖苷（6）、柚皮素（7）、木犀草素（8）。结论化合物1、3和6首次从该属植物中分离得到，化合物2首次从该植物中分离得到。

Chemical Constituents in ethyl acetate fraction from Viola yedoensis

Objective To study the chemical constituents in ethyl acetate fraction from Viola yedoensis.Methods The constituents were isolated by various chromatographic techniques (silica gel,RP-MPLC,and PHPLC)and their structures were determined on the basis of their spectroscopic data,as well as literatures.Results Eight compounds were obtained and identified as quercetin-3-O-β-D-glucosyl-(1 -4)-O-α-L-rhamnoside (1),keampferol-3-O-rutinoside (2),keampferol-3 -O-β-D-glucosyl-(1 -2)-O-α-L-rhamnoside (3),6,7 -dihydroxycoumarin (4),7 -hydroxy-8 -methoxycoumarin (5),6 -hydroxy-7 -O-α-L-rhamnosyl-(6-1)-O-β-D-glucoside]coumarin (6),naringenin (7),and luteolin (8).Conclusion Compounds 1, 3,and 6 were isolated from Viola genus for the first time,and compounds 2 were isolated from this plant for the first time.