茶多酚对胶原诱导性关节炎大鼠足爪组织MMP-2蛋白表达水平的影响 |
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引用本文: | 陈永顺,李静,蒋建平,梁建梅,贾晓栋. 茶多酚对胶原诱导性关节炎大鼠足爪组织MMP-2蛋白表达水平的影响[J]. 安徽医药, 2015, 0(6): 1059-1062. DOI: 10.3969/j.issn.1009-6469.2015.06.011 |
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作者姓名: | 陈永顺 李静 蒋建平 梁建梅 贾晓栋 |
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作者单位: | 1. 商丘医学高等专科学校,河南 商丘,476100;2. 广西医科大学药学院,广西 南宁,530021 |
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基金项目: | 河南省教育厅科学技术研究重点项目 |
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摘 要: | 目的:探讨茶多酚对Ⅱ型胶原乳化剂诱导关节炎大鼠的治疗作用及机制。方法 SD大鼠随机分为正常对照组、模型组、地塞米松对照组(2 mg·kg-1·d-1)和茶多酚治疗组(200 mg·kg-1·d-1),采用Ⅱ型胶原弗氏完全佐剂法(collagen-in-duced arthritis,CIA)建立类风湿性关节炎大鼠模型。记录大鼠足趾体积的变化,酶联免疫法(ELISA)检测大鼠血清IL-1β、PGE2水平,免疫组化法检测大鼠足爪组织基质金属蛋白酶2(MMP-2)蛋白水平的表达。结果与模型组比较,茶多酚治疗组大鼠踝关节肿胀明显减轻(P<0.05),大鼠血清IL-Iβ、PEG2水平明显降低(P<0.05),足爪组织细胞中MMP-2蛋白表达明显下降(P<0.05)。结论茶多酚对CIA大鼠关节炎有抑制作用,其机制可能与抑制炎症介质IL-1β、PEG2,下调MMP-2蛋白表达有关。
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关 键 词: | 茶多酚 胶原诱导性关节炎 基质金属蛋白酶2 |
Effect of Tea Polyphenols on paw MMP-2 protein expression in collagen-induced arthritis rats |
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Abstract: | Objective To investigate the effect of Tea polyphenols on collagen-induced arthritis(CIA)rats,and to explore its therapeutic mechanism.Methods SD rats were randomized into four groups:normal control group,model group,dexamethasone group (2 mg · kg-1 ·d-1 )and tea polyphenols group(200 mg·kg-1 ·d-1 ).The rats were given pedal intradermal injection of collagen and Com-plete Freund's Adjuvant to induce arthritis.We recorded the changes of rats in toe volume .The serum content of IL-1βand PGE2 were assayed by ELISA,and the expression of MMP-2 on rats paw was detected by immunohistochemical staining.Results As compared with the model group,the ankle joint swelling degree was relieved in tea polyphenols group (P<0.05 ),the serum levels of IL-1β&PEG2 were significantly decreased(P<0.05),and the expression of MMP-2 protein on paw also decreased(P<0.05).Conclusions Tea polyphenols has an obvious inhibition on CIA rats,and its mechanism may be related to the reduction of IL-1 β&PEG2 ,and down-regulation of MMP-2. |
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Keywords: | tea polyphenols collagen-induced arthritis MMP-2 |
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